Open Access
ARTICLE
p53 siRNA promotes autophagy of U2OS cells through its target gene Rap2B
Heya QIAN1,§, Yan YAN2,§, Zhengjie SHEN1, Lixian XU1, Yun ZUO1, Tao ZHU3,*, Yanan CHEN1,*
1 Department of Oncology, the Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang, 215600, China
2 Department of Pharmacology, the Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang, 215600, China
3 Department of Laboratory, the Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang, 215600, China
§ Both authors contributed equally to this work.
* Address correspondence to: Tao Zhu,; Yanan Chen,
BIOCELL 2019, 43(4), 321-326. https://doi.org/10.32604/biocell.2019.07992
Abstract
The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS
cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε
were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3
in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca
2+ were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly
decreased the expressions of Rap2B protein. Additionally, knockdown of p53 significantly decreased the mRNA levels
of PLCε. The knockdown of p53, Rap2B, and PLCε significantly decreased the levels of intracellular IP3 and Ca
2+ and promoted autophagy of U2OS cells. Our results demonstrated that p53-Rap2B-PLCε-IP3 signaling pathway regulated
autophagy of U2OS cells.
Keywords
Cite This Article
QIAN, H., YAN, Y., SHEN, Z., XU, L., ZUO, Y. et al. (2019). p53 siRNA promotes autophagy of U2OS cells through its target gene Rap2B.
BIOCELL, 43(4), 321–326.