Open Access

ARTICLE

p53 siRNA promotes autophagy of U2OS cells through its target gene Rap2B

Heya QIAN^1,§, Yan YAN^2,§, Zhengjie SHEN¹, Lixian XU¹, Yun ZUO¹, Tao ZHU^3,*, Yanan CHEN^1,*

1 Department of Oncology, the Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang, 215600, China
2 Department of Pharmacology, the Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang, 215600, China
3 Department of Laboratory, the Affiliated Zhangjiagang Hospital of Soochow University, Zhangjiagang, 215600, China
§ Both authors contributed equally to this work.

* Address correspondence to: Tao Zhu,; Yanan Chen,

BIOCELL 2019, 43(4), 321-326. https://doi.org/10.32604/biocell.2019.07992

Abstract

The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3 in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca²⁺ were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly decreased the expressions of Rap2B protein. Additionally, knockdown of p53 significantly decreased the mRNA levels of PLCε. The knockdown of p53, Rap2B, and PLCε significantly decreased the levels of intracellular IP3 and Ca²⁺ and promoted autophagy of U2OS cells. Our results demonstrated that p53-Rap2B-PLCε-IP3 signaling pathway regulated autophagy of U2OS cells.

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Keywords

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