Open Access

ARTICLE

Association of TRIM22 with the type 1 interferon response during primary human cytomegalovirus infection in THP-1 macrophages

Wei LI, Huihui GAO, Ran TAO, Lifang LIU, Shiqiang SHANG^*

Department of Clinical Laboratory, the Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center For Child Health, Hangzhou, 310052, China

* Address correspondence to: Shiqiang Shang,

BIOCELL 2019, 43(4), 285-291. https://doi.org/10.32604/biocell.2019.08177

Abstract

As a response factor of interferon, tripartite motif (TRIM) 22 was reported to exert antiviral activity against viruses. In this study, THP-1 macrophages were infected with human cytomegalovirus (HCMV) to establish the HCMV lytic infection model. The mRNA levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and interferonbeta (IFN-β) were significantly up-regulated in THP-1 macrophages at different infection time and titers. Moreover, for the first time, upregulation of TRIM22 expression was found during HCMV infection at both mRNA and protein levels in THP-1 macrophages. Furthermore, IFN-β could induce TRIM22 expression in THP-1 macrophages or HCMV infected THP-1 macrophages. Depletion of TRIM22 increased replication activity of HCMV with increasing of HCMV titers and HCMV proteins. In conclusion, it is the first report that HCMV can induce TRIM22 activation through IFN-β signaling and TRIM22 can suppress replication of HCMV in THP-1 macrophages.

---

Keywords

---