Open Access
ARTICLE
An extract of Hypericum perforatum induces wound healing through inhibitions of Ca2+ mobilizations, mitochondrial oxidative stress and cell death in epithelial cells: Involvement of TRPM2 channels
Fuat USLUSOY1, Mustafa NAZIROĞLU2,3,*
1 Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey
2 Neuroscience Research Center, Suleyman Demirel University, Isparta, Turkey
3 Drug Discovery Unit, BSN Health, Analysis and Innovation Ltd. Inc. Teknokent, Isparta, Turkey
* Address correspondence to: Mustafa NAZIROĞLU,
BIOCELL 2019, 43(4), 271-283. https://doi.org/10.32604/biocell.2019.08333
Abstract
The wound is induced by several mechanical and metabolic factors. In the etiology of the wound recovery,
excessive oxidative stress, calcium ion (Ca
2+) influx, and apoptosis have important roles. Ca
2+-permeable TRPM2 channel is activated by oxidative stress. Protective roles of
Hypericum perforatum extract (HP) on the mechanical nerve injury-induced apoptosis and oxidative toxicity through regulation of TRPM2 in the experimental animals were
recently reported. The potential protective roles in HP treatment were evaluated on the TRPM2-mediated cellular
oxidative toxicity in the renal epithelium (MPK) cells. The cells were divided into three groups as control, wound,
and wound + HP treatment (75 µM for 72 h). Wound diameters were more importantly decreased in the wound+HP
group than in the wound group. In addition, the results of laser confocal microscopy analyses indicated protective
roles of HP and TRPM2 antagonists (N-(p-Amylcinnamoyl) anthranilic acid and 2-aminoethyl diphenylborinate)
against the wound-induced increase of Ca
2+ influx and mitochondrial ROS production. The wound-induced increase
of early (annexin V-FITC) apoptosis and late (propidium iodide) apoptosis were also decreased in the cells by the HP
treatment. In conclusion, HP treatment acted protective effects against wound-mediated oxidative cell toxicity and
apoptosis through TRPM2 inhibition. These effects may be attributed to their potent antioxidant effect.
Keywords
Cite This Article
USLUSOY, F., NAZIROĞLU, M. (2019). An extract of
Hypericum perforatum induces wound healing through inhibitions of Ca
2+ mobilizations, mitochondrial oxidative stress and cell death in epithelial cells: Involvement of TRPM2 channels.
BIOCELL, 43(4), 271–283.
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