Open Access

ARTICLE

Shuzhen SHI^{1, 2}, Ming LIU^{1, 2}, Wenjuan MA^{1, 2}, Ya GAO^{1, 2}, Long GE³, Xiping SHEN³, Jiarui WU⁴, Junhua ZHANG^{5, *}, Jinhui TIAN^{1, 2, *}
BIOCELL, Vol.43, No.4, pp. 233-251, 2019, DOI:10.32604/biocell.2019.07624
Abstract The study aimed to analyze the reporting and methodological quality of systematic reviews (SRs)/metaanalyses (MAs) of animal models to provide references for later studies and avoid the waste of medical resources. EMBASE and MEDLINE databases were searched from inception to November 2017, with no language restriction. Two reviewers selected inclusion dependently and extracted the basic characteristics. Review Manager 5.3, stata 12.0, and SPSS 21 software were used to conduct analyses. A total of 46 SRs/MAs were included. The results showed that the English databases with high retrieval frequency are PubMed/MEDLINE, EMBASE, and Web of Science.… More >

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1862

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REVIEW

Tarek A ATIA
BIOCELL, Vol.43, No.4, pp. 253-262, 2019, DOI:10.32604/biocell.2019.08180
Abstract Recurring miscarriage (RM) is a frustrating reproductive complication with variable etiology. Numerous genetic defects have been known to play a crucial role in the etiology of RM. Chromosomal abnormalities are frequently detected, while other genetic defects cannot be diagnosed through routine research, such as cryptic chromosomal anomalies, single nucleotide polymorphism, single-gene defect, and gene copy number variation. Diagnostic laboratories have recently used variable advanced techniques to detect potential genetic abnormalities in couples with RM and/or in products of conception. Here we aim to summarize the known genetic causes of RM, with a focus on the More >

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2348

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1

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ARTICLE

Yuan LI¹, Yonghyun LEE², Yielhea SEO³, Youjin HWANG^{1, 2, *}
BIOCELL, Vol.43, No.4, pp. 263-269, 2019, DOI:10.32604/biocell.2019.07664
Abstract The aim of this study was to determine the relationship between phenotypic antimicrobial susceptibility patterns and extended-spectrum, carbapenem-resistance genes. A total of 109 clinical Staphilococcus aureus strains were subjected to 19 antimicrobial susceptibility tests. Resistance to methicillin (mecA), penicillin (blaTEM), and tetracycline (tetM) was detected. We compared the presence of the blaTEM genes with extended-spectrum, carbapenem-related genes and identified the types of SCCmec genes. Of 109 clinical S. aureus strains, 62 (56.88%) had methicillin resistance and 60 strains carried mecA. The prevalence of blaTEM and tetM genes was 81.65% and 37.61%, respectively. The most predominant SCCmec type More >

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2036

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1513

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1

Open Access

ARTICLE

Fuat USLUSOY¹, Mustafa NAZIROĞLU^2,3,*
BIOCELL, Vol.43, No.4, pp. 271-283, 2019, DOI:10.32604/biocell.2019.08333
Abstract The wound is induced by several mechanical and metabolic factors. In the etiology of the wound recovery, excessive oxidative stress, calcium ion (Ca²⁺) influx, and apoptosis have important roles. Ca²⁺-permeable TRPM2 channel is activated by oxidative stress. Protective roles of Hypericum perforatum extract (HP) on the mechanical nerve injury-induced apoptosis and oxidative toxicity through regulation of TRPM2 in the experimental animals were recently reported. The potential protective roles in HP treatment were evaluated on the TRPM2-mediated cellular oxidative toxicity in the renal epithelium (MPK) cells. The cells were divided into three groups as control, wound, and wound… More >

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2012

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1683

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2

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ARTICLE

Wei LI, Huihui GAO, Ran TAO, Lifang LIU, Shiqiang SHANG^*
BIOCELL, Vol.43, No.4, pp. 285-291, 2019, DOI:10.32604/biocell.2019.08177
Abstract As a response factor of interferon, tripartite motif (TRIM) 22 was reported to exert antiviral activity against viruses. In this study, THP-1 macrophages were infected with human cytomegalovirus (HCMV) to establish the HCMV lytic infection model. The mRNA levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and interferonbeta (IFN-β) were significantly up-regulated in THP-1 macrophages at different infection time and titers. Moreover, for the first time, upregulation of TRIM22 expression was found during HCMV infection at both mRNA and protein levels in THP-1 macrophages. Furthermore, IFN-β could induce TRIM22 expression in THP-1 macrophages or HCMV More >

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2127

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1588

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ARTICLE

Tiangen WU¹, Tianhao BAO^2,3, Daoming LIANG^1,*, Lin WANG^4,*
BIOCELL, Vol.43, No.4, pp. 293-298, 2019, DOI:10.32604/biocell.2019.07841
Abstract Circulating tumor cells (CTCs) are crucial to tumor metastasis, and they usually undergo epithelial– mesenchymal transition (EMT) in order to disseminate from the primary tumor. However, very little is currently known about the relationship between EMT and malignant phenotypes of CTCs in the context of gastric cancer. Therefore, this study aimed to investigate the contribution of EMT to malignant phenotypes of CTCs derived from gastric cancer cells. We xenografted MKN28 gastric cancer cells pretreated with transforming growth factor-beta 1 (TGFβ-1) into nude mice by intravenous injection. Next, we isolated CTCs from the blood of nude… More >

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1623

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4

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ARTICLE

Denis BARANENKO¹, Julia FEDOTOVA^{1, 2, *}, Annemieke Johanna Maria VAN DEN TOL³
BIOCELL, Vol.43, No.4, pp. 299-311, 2019, DOI:10.32604/biocell.2019.08407
Abstract The impact of various vitamin D3 (VD3) doses (1.0, 2.5, or 5 mg/kg, s.c.) in mitigating the negative consequences of chronic unpredictable mild stress (CUMS) was investigated. Adult female rats with long-term estrogen deficiency were assessed using the sucrose preference test (SPT), the elevated plus-maze (EPM), the light/dark test (LDT), and the open-field test (OFT) to measure anhedonia-like and anxiety-like behavior. The corticosterone (CS) and adrenocorticotrophic hormone (ACTH) concentrations in blood serum and the brain-derived neurotrophic factor (BDNF) expression in the hippocampus of long-term ovariectomized (OVX) rats were measured by ELISA kits and/ or western… More >

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2215

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1732

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ARTICLE

Xiuchun ZHANG
BIOCELL, Vol.43, No.4, pp. 313-319, 2019, DOI:10.32604/biocell.2019.07973
Abstract To investigate the effects of polydatin on the proliferation, migration, and invasion of ovarian cancer, the change of proliferative ability, migration ability, and invasive ability of human ovarian cancer cell OVCAR-3, A2780, and HO-8910 was detected by using polydatin and up-regulating PI3K. The anticancer activity and mechanism of polydatin in ovarian cancer were analyzed. Polydatin could effectively inhibit the proliferation, migration, and invasion of OVCAR-3, A2780, and HO-8910, and inhibit the expression of PI3K protein. After the expression level of PI3K protein was up-regulated, the inhibitory effect of polydatin on the proliferative ability, migration ability, More >

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1792

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ARTICLE

Heya QIAN^1,§, Yan YAN^2,§, Zhengjie SHEN¹, Lixian XU¹, Yun ZUO¹, Tao ZHU^3,*, Yanan CHEN^1,*
BIOCELL, Vol.43, No.4, pp. 321-326, 2019, DOI:10.32604/biocell.2019.07992
Abstract The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3 in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca²⁺ were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly decreased the expressions of Rap2B protein. Additionally, knockdown of p53 More >

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1951

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1561

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ARTICLE

Mohammed Fadhil HADDAD^1,2,3, Tugba DAYIOGLU^1,2, Barbaros NALBANTOĞLU², Turgay CAKMAK^1,*
BIOCELL, Vol.43, No.4, pp. 327-333, 2019, DOI:10.32604/biocell.2019.08522
Abstract Cyanobacteria are considered as a sustainable feedstock for the production of biochemically active compounds such as phycobiliproteins (PBPs). In this study, the impact of nitrogen (N) and phosphorus (P) availability on PBP production of “N-free acclimated” Anabaena variabilis IMU8 was analyzed. Upon isolation and identification, the cyanobacterium has been maintained in N-free BG-11 medium for more than 20 months. For experimentation, the strain was incubated in N-replete, N-depleted, N-P-depleted BG-11 medium. Long-term diazotrophic cultivation of A. variabilis IMU8 resulted in elevated PBP productivity with a limited impact on growth. When compared to N-depleted ones, N supply stimulated More >

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1566