Open Access
ARTICLE
Shuzhen SHI1, 2, Ming LIU1, 2, Wenjuan MA1, 2, Ya GAO1, 2, Long GE3, Xiping SHEN3, Jiarui WU4, Junhua ZHANG5, *, Jinhui TIAN1, 2, *
BIOCELL, Vol.43, No.4, pp. 233-251, 2019, DOI:10.32604/biocell.2019.07624
Abstract The study aimed to analyze the reporting and methodological quality of systematic reviews (SRs)/metaanalyses
(MAs) of animal models to provide references for later studies and avoid the waste of medical resources.
EMBASE and MEDLINE databases were searched from inception to November 2017, with no language restriction.
Two reviewers selected inclusion dependently and extracted the basic characteristics. Review Manager 5.3, stata
12.0, and SPSS 21 software were used to conduct analyses. A total of 46 SRs/MAs were included. The results showed
that the English databases with high retrieval frequency are PubMed/MEDLINE, EMBASE, and Web of Science.
67.31% (31/46) of the articles… More >
Open Access
REVIEW
Tarek A ATIA
BIOCELL, Vol.43, No.4, pp. 253-262, 2019, DOI:10.32604/biocell.2019.08180
Abstract Recurring miscarriage (RM) is a frustrating reproductive complication with variable etiology. Numerous
genetic defects have been known to play a crucial role in the etiology of RM. Chromosomal abnormalities are
frequently detected, while other genetic defects cannot be diagnosed through routine research, such as cryptic
chromosomal anomalies, single nucleotide polymorphism, single-gene defect, and gene copy number variation.
Diagnostic laboratories have recently used variable advanced techniques to detect potential genetic abnormalities in
couples with RM and/or in products of conception. Here we aim to summarize the known genetic causes of RM, with
a focus on the new diagnostic techniques. Knowledge of… More >
Open Access
ARTICLE
Yuan LI1, Yonghyun LEE2, Yielhea SEO3, Youjin HWANG1, 2, *
BIOCELL, Vol.43, No.4, pp. 263-269, 2019, DOI:10.32604/biocell.2019.07664
Abstract The aim of this study was to determine the relationship between phenotypic antimicrobial susceptibility
patterns and extended-spectrum, carbapenem-resistance genes. A total of 109 clinical Staphilococcus aureus strains were
subjected to 19 antimicrobial susceptibility tests. Resistance to methicillin (mecA), penicillin (blaTEM), and tetracycline
(tetM) was detected. We compared the presence of the blaTEM genes with extended-spectrum, carbapenem-related
genes and identified the types of SCCmec genes. Of 109 clinical S. aureus strains, 62 (56.88%) had methicillin resistance
and 60 strains carried mecA. The prevalence of blaTEM and tetM genes was 81.65% and 37.61%, respectively. The most
predominant SCCmec type was SCCmec type… More >
Open Access
ARTICLE
Fuat USLUSOY1, Mustafa NAZIROĞLU2,3,*
BIOCELL, Vol.43, No.4, pp. 271-283, 2019, DOI:10.32604/biocell.2019.08333
Abstract The wound is induced by several mechanical and metabolic factors. In the etiology of the wound recovery,
excessive oxidative stress, calcium ion (Ca2+) influx, and apoptosis have important roles. Ca2+-permeable TRPM2 channel is activated by oxidative stress. Protective roles of Hypericum perforatum extract (HP) on the mechanical nerve injury-induced apoptosis and oxidative toxicity through regulation of TRPM2 in the experimental animals were
recently reported. The potential protective roles in HP treatment were evaluated on the TRPM2-mediated cellular
oxidative toxicity in the renal epithelium (MPK) cells. The cells were divided into three groups as control, wound,
and wound + HP treatment… More >
Open Access
ARTICLE
Wei LI, Huihui GAO, Ran TAO, Lifang LIU, Shiqiang SHANG*
BIOCELL, Vol.43, No.4, pp. 285-291, 2019, DOI:10.32604/biocell.2019.08177
Abstract As a response factor of interferon, tripartite motif (TRIM) 22 was reported to exert antiviral activity against
viruses. In this study, THP-1 macrophages were infected with human cytomegalovirus (HCMV) to establish the HCMV
lytic infection model. The mRNA levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and interferonbeta
(IFN-β) were significantly up-regulated in THP-1 macrophages at different infection time and titers. Moreover,
for the first time, upregulation of TRIM22 expression was found during HCMV infection at both mRNA and protein
levels in THP-1 macrophages. Furthermore, IFN-β could induce TRIM22 expression in THP-1 macrophages or HCMV
infected THP-1 macrophages. Depletion of… More >
Open Access
ARTICLE
Tiangen WU1, Tianhao BAO2,3, Daoming LIANG1,*, Lin WANG4,*
BIOCELL, Vol.43, No.4, pp. 293-298, 2019, DOI:10.32604/biocell.2019.07841
Abstract Circulating tumor cells (CTCs) are crucial to tumor metastasis, and they usually undergo epithelial–
mesenchymal transition (EMT) in order to disseminate from the primary tumor. However, very little is currently
known about the relationship between EMT and malignant phenotypes of CTCs in the context of gastric cancer.
Therefore, this study aimed to investigate the contribution of EMT to malignant phenotypes of CTCs derived from
gastric cancer cells. We xenografted MKN28 gastric cancer cells pretreated with transforming growth factor-beta 1
(TGFβ-1) into nude mice by intravenous injection. Next, we isolated CTCs from the blood of nude mice by gradient
centrifugation and… More >
Open Access
ARTICLE
Denis BARANENKO1, Julia FEDOTOVA1, 2, *, Annemieke Johanna Maria VAN DEN TOL3
BIOCELL, Vol.43, No.4, pp. 299-311, 2019, DOI:10.32604/biocell.2019.08407
Abstract The impact of various vitamin D3 (VD3) doses (1.0, 2.5, or 5 mg/kg, s.c.) in mitigating the negative
consequences of chronic unpredictable mild stress (CUMS) was investigated. Adult female rats with long-term estrogen
deficiency were assessed using the sucrose preference test (SPT), the elevated plus-maze (EPM), the light/dark test
(LDT), and the open-field test (OFT) to measure anhedonia-like and anxiety-like behavior. The corticosterone (CS)
and adrenocorticotrophic hormone (ACTH) concentrations in blood serum and the brain-derived neurotrophic factor
(BDNF) expression in the hippocampus of long-term ovariectomized (OVX) rats were measured by ELISA kits and/
or western blotting. Treatment with VD3 (5.0… More >
Open Access
ARTICLE
Xiuchun ZHANG
BIOCELL, Vol.43, No.4, pp. 313-319, 2019, DOI:10.32604/biocell.2019.07973
Abstract To investigate the effects of polydatin on the proliferation, migration, and invasion of ovarian cancer, the
change of proliferative ability, migration ability, and invasive ability of human ovarian cancer cell OVCAR-3, A2780,
and HO-8910 was detected by using polydatin and up-regulating PI3K. The anticancer activity and mechanism of
polydatin in ovarian cancer were analyzed. Polydatin could effectively inhibit the proliferation, migration, and invasion
of OVCAR-3, A2780, and HO-8910, and inhibit the expression of PI3K protein. After the expression level of PI3K
protein was up-regulated, the inhibitory effect of polydatin on the proliferative ability, migration ability, and invasive
ability of OVCAR-3,… More >
Open Access
ARTICLE
Heya QIAN1,§, Yan YAN2,§, Zhengjie SHEN1, Lixian XU1, Yun ZUO1, Tao ZHU3,*, Yanan CHEN1,*
BIOCELL, Vol.43, No.4, pp. 321-326, 2019, DOI:10.32604/biocell.2019.07992
Abstract The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS
cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε
were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3
in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca2+ were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly
decreased the expressions of Rap2B protein. Additionally, knockdown of p53 significantly decreased the mRNA… More >
Open Access
ARTICLE
Mohammed Fadhil HADDAD1,2,3, Tugba DAYIOGLU1,2, Barbaros NALBANTOĞLU2, Turgay CAKMAK1,*
BIOCELL, Vol.43, No.4, pp. 327-333, 2019, DOI:10.32604/biocell.2019.08522
Abstract Cyanobacteria are considered as a sustainable feedstock for the production of biochemically active
compounds such as phycobiliproteins (PBPs). In this study, the impact of nitrogen (N) and phosphorus (P) availability
on PBP production of “N-free acclimated” Anabaena variabilis IMU8 was analyzed. Upon isolation and identification,
the cyanobacterium has been maintained in N-free BG-11 medium for more than 20 months. For experimentation, the
strain was incubated in N-replete, N-depleted, N-P-depleted BG-11 medium. Long-term diazotrophic cultivation of A.
variabilis IMU8 resulted in elevated PBP productivity with a limited impact on growth. When compared to N-depleted
ones, N supply stimulated a slight induction… More >
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