Open Access
ARTICLE
Study of phylogenic relationships of some wild and crop species of Iranian Crocus by ITS nuclear loci
Farshid GOLSHANI1, Barat Ali FAKHERI1, Mahmood SOLOUKI1, Nafiseh MAHDINEZHAD1, Majid Reza KIANI FERIZ2
1
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran
2
Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran
* Address correspondence to: Farshid Golshani,
BIOCELL 2019, 43(3), 225-232. https://doi.org/10.32604/biocell.2019.07547
Abstract
ITS (Internal Transcription Spacer of nuclear ribosomal DNA) regions method was applied to identify and
study the phylogenetic relationships existing between 41 individuals of Crocus, including 8 species collected in Iran (4
crop and 8 wild individuals) and 16 species (2 crop and 27 wild individuals) already registered in GenBank (NCBI).
PCR amplifications were performed using the primer designed on the nucleotide sequence of nuclear region (ITS).
A total of 12 sequences were obtained and registered in NCBI database. In particular, 4 of these sequences were not
already present in the scientific library. Nucleotide polymorphic sites were counted for ITS (N = 97). The results showed
that, the Cytosine base is the most abundant nucleotide. Relatively high conservation was observed in the ITS regions
of the populations (0.761). Each sample could be distinguished from the others in the phylogenic trees developed
based on the data obtained by ITS barcode genes. In general, ITS sequence indicating high resolution at the species
level is very suitable for phylogenetic studies in the Crocus family. Phylogenetic analysis demonstrated the genetic
relationship between crop saffron and wild Crocus species. According to the results of this study, the wild species of
C.
cartwrightianus and C. oreocreticus were considered as the closest relatives of the saffron. The results of the study also
indicated that, the different ecotypes of
C. sativus may have evolved through independent events probably due to the
geographic and environmental pressures
Keywords
Cite This Article
APA Style
GOLSHANI1, F., FAKHERI, B.A., SOLOUKI, M., MAHDINEZHAD, N., FERIZ, M.R.K. (2019). Study of phylogenic relationships of some wild and crop species of iranian crocus by ITS nuclear loci. BIOCELL, 43(3), 225-232. https://doi.org/10.32604/biocell.2019.07547
Vancouver Style
GOLSHANI1 F, FAKHERI BA, SOLOUKI M, MAHDINEZHAD N, FERIZ MRK. Study of phylogenic relationships of some wild and crop species of iranian crocus by ITS nuclear loci. BIOCELL . 2019;43(3):225-232 https://doi.org/10.32604/biocell.2019.07547
IEEE Style
F. GOLSHANI1, B.A. FAKHERI, M. SOLOUKI, N. MAHDINEZHAD, and M.R.K. FERIZ "Study of phylogenic relationships of some wild and crop species of Iranian Crocus by ITS nuclear loci," BIOCELL , vol. 43, no. 3, pp. 225-232. 2019. https://doi.org/10.32604/biocell.2019.07547