Open Access
ARTICLE
Identification of phytohormone changes and its related genes under abiotic stresses in transgenic rice
Mohammad UBAIDILLAH1,2, Miswar FAPERTA1,2, Kyung-Min KIM1
1
School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University, Daegu, 41566, Korea
2
Department of Agronomy, Faculty of Agriculture, Jember University, Jl. Kalimantan 37, Jember, 68121, Indonesia
* Address correspondence to: Kyung-Min Kim,
BIOCELL 2019, 43(3), 215-224. https://doi.org/10.32604/biocell.2019.07549
Abstract
Abiotic stresses, such as drought and salinity, adversely affect plant growth and productivity. Comparison
between non transgenic and transgenic rice harboring
CaMsrB2 gene, which induces tolerance to abiotic stress, is
important to observe response of gene under abiotic stress. Phytohormone showed a tendency to increase under
the drought stress or salinity stress in the transgenic plant. RT-PCR analysis showed that gene expression and
phytohormone levels under abiotic stress, to be closely related. The
CaMsrB2 gene is related to the expression of JA and
ABA hormones. Therefore, the level of expression of these genes and hormones was observed. The transcription levels
of LOX2 and OsWRKY45 were substantially higher in the wild type rice in comparison to the transformants, which
suggested that phytohormone are also required for the regulation of leaf and root. Comparison between control and
transgenic rice overexpressing a
CaMsrB2 gene, resulted in different pattern of ABA, JA levels under different stress
condition. In both drought and salinity stresses, the expression of OsWRKY45 gene was similar in both treatments with
time. These results suggest that gene involved in the plant physiology response in mechanism to abiotic stress.
Keywords
Cite This Article
UBAIDILLAH, M., FAPERTA, M., KIM, K. (2019). Identification of phytohormone changes and its related genes under abiotic stresses in transgenic rice.
BIOCELL, 43(3), 215–224. https://doi.org/10.32604/biocell.2019.07549