Open Access

ARTICLE

Down-regulation of Halr1 during induced differentiation of embryonal carcinoma P19 cells

Zahra HOSSEININIA¹, Sara SOLTANIAN², Naser MAHDAVI-SHAHRI³, Hesam DEHGHANI^1,4

1 Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2 Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
3 Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
4 Stem Cells and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

* Address correspondence to: Hesam Dehghani,

BIOCELL 2019, 43(3), 145-154. https://doi.org/10.32604/biocell.2019.06468

Abstract

Maintenance of pluripotency depends to diverse regulatory factors. Studies in embryonic stem cells (ESCs) have indicated that large intergenic non-coding RNAs (lincRNAs) are involved in the regulatory network of pluripotency. However, the presence and function of pluripotency-associated lincRNAs in cancer cells with pluripotency features are unknown. In this study, we used embryonal carcinoma (EC) P19 cell lines to investigate the expression level of Halr1 in pluripotency and retinoic acid (RA)-induced differentiated states. Down-regulation of pluripotency associated factors such as OCT4, NANOG, SSEA1 and alkaline phosphatase at transcript and protein levels were used to confirm the differentiated status of P19 cells. Quantitative measurement of Halr1 transcript levels revealed a 79% decrease during RA-induced differentiation of P19 cells. These results indicate that upon exiting the pluripotency state the expression level of Halr1 similar to core pluripotency factors is remarkably reduced.

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Keywords

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