Open Access
ARTICLE
Down-regulation of Halr1 during induced differentiation of embryonal carcinoma P19 cells
Zahra HOSSEININIA1, Sara SOLTANIAN2, Naser MAHDAVI-SHAHRI3, Hesam DEHGHANI1,4
1
Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
3
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
4
Stem Cells and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
* Address correspondence to: Hesam Dehghani,
BIOCELL 2019, 43(3), 145-154. https://doi.org/10.32604/biocell.2019.06468
Abstract
Maintenance of pluripotency depends to diverse regulatory factors. Studies in embryonic stem cells
(ESCs) have indicated that large intergenic non-coding RNAs (lincRNAs) are involved in the regulatory network
of pluripotency. However, the presence and function of pluripotency-associated lincRNAs in cancer cells with
pluripotency features are unknown. In this study, we used embryonal carcinoma (EC) P19 cell lines to investigate the
expression level of Halr1 in pluripotency and retinoic acid (RA)-induced differentiated states. Down-regulation of
pluripotency associated factors such as OCT4, NANOG, SSEA1 and alkaline phosphatase at transcript and protein
levels were used to confirm the differentiated status of P19 cells. Quantitative measurement of Halr1 transcript levels
revealed a 79% decrease during RA-induced differentiation of P19 cells. These results indicate that upon exiting the
pluripotency state the expression level of Halr1 similar to core pluripotency factors is remarkably reduced.
Keywords
Cite This Article
HOSSEININIA, Z., SOLTANIAN, S., MAHDAVI-SHAHRI, N., DEHGHANI, H. (2019). Down-regulation of Halr1 during induced differentiation of embryonal carcinoma P19 cells.
BIOCELL, 43(3), 145–154.