Open Access
ARTICLE
XRCC1 Arg399Gln and Arg194Trp polymorphisms regulate XRCC1 expression and chemoresistance of non-small cell lung cancer cells
Dairong LI1, Xianlu ZHUO1,2, Lumi HUANG1, Xiaohui JI1, Donglin WANG1
1
Chongqing University Cancer Hospital & Chongqing Cancer Institute & Chongqing Cancer Hospital, Chongqing, 400030, China
2
Postdoctoral Scientific Research Station, Chongqing Cancer Institute, Chongqing, 400030, China
* Address correspondence to: Dairong Li,
BIOCELL 2019, 43(3), 139-144. https://doi.org/10.32604/biocell.2019.06460
Abstract
X-ray repair cross-complementing protein 1 (XRCC1) could repair cisplatin-induced DNA damage. XRCC1
Arg399Gln and Arg194Trp variants alter XRCC1 expression and function, leading to changes in cancer sensitivity to
cisplatin treatment. This study aimed to investigate the effects of XRCC1 Arg399Gln and Arg194Trp polymorphisms
on cell viability, apoptosis and XRCC1 expression in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP nonsmall cell lung cancer (NSCLC) cells. Plasmids carrying XRCC1 Arg399Gln and Arg194Trp were constructed and
transfected into A549 and A549/DDP cells. RT–PCR, Western blot, MTT assay, and flow cytometry analysis were
performed to assess cell viability, apoptosis, and XRCC1 expression. Compared to control cells, the viability of A549
and A549/DDP cells transfected with XRCC1 Arg399Gln and Arg194Trp was higher and the apoptosis rate was lower,
and XRCC1 mRNA and protein expression levels were significantly higher. In conclusion, our results suggest that
XRCC1 Arg399Gln and Arg194Trp polymorphisms change XRCC1 expression in NSCLC cells and alter the sensitivity
of NSCLC to cisplatin-based chemotherapy
Keywords
Cite This Article
LI, D., ZHUO, X., HUANG, L., JI, X., WANG, D. (2019).
XRCC1 Arg399Gln and Arg194Trp polymorphisms regulate XRCC1 expression and chemoresistance of non-small cell lung cancer cells.
BIOCELL, 43(3), 139–144.