TY - EJOU
AU - MOUSTAFA, Mahmoud
AU - ALAMRI, Saad
AU - ZAKI, Hoida
AU - LOUTFY, Naglaa
AU - TAHA, Tarek
AU - SHATI, Ali
AU - ALKAHTANI, Mohamed
AU - SIDDIQI, Sajda
TI - Gene expression of 49 kDa apyrase, cytoskeletal proteins, ATPase, ADPase and amino acid contents of Pisum sativum (L.) cells germinated in Euryops arabicus (Steud. ex Jaub. \& Spach) water extract
T2 - BIOCELL
PY - 2019
VL - 43
IS - 2
SN - 1667-5746
AB - The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the
gene expression of 49-kDa apyrases, cytoskeletal proteins, ATPases, ADPase and amount of amino acid of pea (Pisum
sativum L. var. Alaska). Pellets of cytoskeletals proteins (27000 xg) were probed with anti-apyrase antibody, biotinylated
anti-rat, actin and alpha and beta-tubulin for Western blotting. ATPase and ADPase activities were determined based
on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate. By 72 hours, the abundance of apyrases,
cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds in E. arabicus extracts
were sharply increased than those sown in distilled water. All the samples exhibited that the stems had more amount
from apyrases, cytoskeletal proteins, amino acids and ATPase and ADPase activities than primary leaves and primary
roots that were germinated either on E. arabicus water extract or in distilled water. Based on the enzyme’s capability to
hydrolyse nucleotide triphosphate and nucleotide diphosphate as well as the direct association between expression of
49-kDa apyrase and cytoskeletal proteins, E. arabicus water extract had an important effect on plant germinations.
KW - Cytoskeletal proteins
KW - ATPase
KW - ADPase
KW - Pisum sativum
KW - Euryops arabicus
DO - 10.32604/biocell.2019.06231