Open Access

ARTICLE

Gene expression of 49 kDa apyrase, cytoskeletal proteins, ATPase, ADPase and amino acid contents of Pisum sativum (L.) cells germinated in Euryops arabicus (Steud. ex Jaub. & Spach) water extract

Mahmoud MOUSTAFA^1,2, Saad ALAMRI¹, Hoida ZAKI², Naglaa LOUTFY², Tarek TAHA³, Ali SHATI¹, Mohamed ALKAHTANI¹, Sajda SIDDIQI¹

1 Department of Biology, College of Science, King Khalid University, 9004, Abha, Kingdom of Saudi Arabia (KSA)
2 Department of Botany, Faculty of Science, South Valley University, Qena, Egypt
3 Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt

* Address correspondence to: Mahmoud MOUSTAFA,

BIOCELL 2019, 43(2), 81-88. https://doi.org/10.32604/biocell.2019.06231

Abstract

The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases, cytoskeletal proteins, ATPases, ADPase and amount of amino acid of pea (Pisum sativum L. var. Alaska). Pellets of cytoskeletals proteins (27000 xg) were probed with anti-apyrase antibody, biotinylated anti-rat, actin and alpha and beta-tubulin for Western blotting. ATPase and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate. By 72 hours, the abundance of apyrases, cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds in E. arabicus extracts were sharply increased than those sown in distilled water. All the samples exhibited that the stems had more amount from apyrases, cytoskeletal proteins, amino acids and ATPase and ADPase activities than primary leaves and primary roots that were germinated either on E. arabicus water extract or in distilled water. Based on the enzyme’s capability to hydrolyse nucleotide triphosphate and nucleotide diphosphate as well as the direct association between expression of 49-kDa apyrase and cytoskeletal proteins, E. arabicus water extract had an important effect on plant germinations.

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Keywords

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