Open Access

ARTICLE

The expressional level of tankyrase-1 gene and its regulation in colorectal cancer in a Saudi population

by Hala Abdulaziz M ALWARTHAN, Mohammad Saud AL ANAZI, Narasimha R. PARINE, Ramesa Shafi BHAT, Ghadah ALAMRO, Ftoon ALJARBOU, Sooad K AL-DAIHAN

1 Department of Biochemistry, College of Science, King Saud University, P. O. Box 22452, Zip code 11495, Riyadh, Saudi Arabia

* Address correspondence to: Ramesa Shafi Bhat,

BIOCELL 2019, 43(2), 51-58. https://doi.org/10.32604/biocell.2019.07019

Abstract

Tankyrase1 plays an essential role in cancer progression by regulating telomere length. The study aimed to determine expression of TNKS1 and its regulation in colorectal cancer (CRC) in 20 samples from Saudi patients. mRNA expression of TNKS1 in CRC and paired normal tissues was measured by qRT-PCR. Epigenetic modification of TNKS1 promoter was determined by methylation-specific PCR while somatic mutation was analyzed by Sanger sequencing in exon 10 of the gene. All cancerous and normal tissues expressed TNKS1, but level of expression in CRC tissues was significantly associated with tumor stage though no other parameters; age, gender, and tumor location, showed any correlation. Expression of TNKS1 was markedly higher in earlier (I, II) than later (III, IV) stages of CRC development. Both cancerous and healthy tissues had unmethylated promoter. Sanger sequencing of exon 10 masked any somatic mutation in the samples. Our findings suggest that up-regulation of TNKS1 was inversely correlated with cancer progression in CRC, indicating that TNKS1 participates in the initiation of CRC by stabilizing telomere length in the first phase of cancer progression. Mechanisms other than TNKS1 might play a role in malignant tumor progression and telomere maintenance in the late stages of CRC.

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