Open Access
ARTICLE
The expressional level of tankyrase-1 gene and its regulation in colorectal cancer in a Saudi population
Hala Abdulaziz M ALWARTHAN1, Mohammad Saud AL ANAZI1, Narasimha R. PARINE1, Ramesa Shafi BHAT1,*, Ghadah ALAMRO1, Ftoon ALJARBOU1, Sooad K AL-DAIHAN1
1
Department of Biochemistry, College of Science, King Saud University, P. O. Box 22452, Zip code 11495, Riyadh, Saudi Arabia
* Address correspondence to: Ramesa Shafi Bhat,
BIOCELL 2019, 43(2), 51-58. https://doi.org/10.32604/biocell.2019.07019
Abstract
Tankyrase1 plays an essential role in cancer progression by regulating telomere length. The study aimed
to determine expression of
TNKS1 and its regulation in colorectal cancer (CRC) in 20 samples from Saudi patients.
mRNA expression of
TNKS1 in CRC and paired normal tissues was measured by qRT-PCR. Epigenetic modification
of
TNKS1 promoter was determined by methylation-specific PCR while somatic mutation was analyzed by Sanger
sequencing in exon 10 of the gene. All cancerous and normal tissues expressed
TNKS1, but level of expression in CRC
tissues was significantly associated with tumor stage though no other parameters; age, gender, and tumor location,
showed any correlation. Expression of
TNKS1 was markedly higher in earlier (I, II) than later (III, IV) stages of CRC
development. Both cancerous and healthy tissues had unmethylated promoter. Sanger sequencing of exon 10 masked
any somatic mutation in the samples. Our findings suggest that up-regulation of
TNKS1 was inversely correlated with
cancer progression in CRC, indicating that
TNKS1 participates in the initiation of CRC by stabilizing telomere length in
the first phase of cancer progression. Mechanisms other than
TNKS1 might play a role in malignant tumor progression
and telomere maintenance in the late stages of CRC.
Keywords
Cite This Article
Abdulaziz, H., Saud, M., PARINE, N. R., BHAT, R. S., ALAMRO, G. et al. (2019). The expressional level of tankyrase-1 gene and its regulation in colorectal cancer in a Saudi population.
BIOCELL, 43(2), 51–58. https://doi.org/10.32604/biocell.2019.07019