Open Access

ARTICLE

Hydrothermal preparation of TiO₂-Ag nanoparticles and its antimicrobial performance against human pathogenic microbial cells in water

Mahmoud MOUSTAFA^{1, 2}, Saad ALAMRI¹, Mohamed ELNOUBY³, Tarek TAHA⁴, M. A. ABU-SAIED⁵, Ali SHATI¹, Mohamed AL-KAHTANI¹, Sulaiman ALRUMMAN¹

1 Department of Biology, College of Science, King Khalid University, 9004, Abha, Kingdom of Saudi Arabia (KSA)
2 Department of Botany, Faculty of Science, South Valley University, Qena, Egypt
3 Composite and Nanostructured Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-CITY), New Borg El-Arab City, 21934, Alexandria, Egypt
4 Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-CITY), New Borg El-Arab City, 21934, Alexandria, Egypt
5 Polymer Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-CITY), New Borg El-Arab City, 21934, Alexandria, Egypt

* Address correspondence to: Mahmoud MOUSTAFA,

BIOCELL 2018, 42(3), 93-98. https://doi.org/10.32604/biocell.2018.07014

Abstract

Water contaminated with pathogenic microbes is considered as one of the most common routes for transmitting diseases in human beings. Different methods have been applied for the decontamination of microbes in contaminated water. In the current study, an easy to do hydrothermal method has been used for the preparation of TiO₂-Ag nanoparticles. The obtained material was characterised using a scanning electron microscope (SEM) and fourier transform infra-red spectroscopy (FTIR). The morphological appearance of the obtained nanoparticles was in the shape of a sphere with a size range of 60-90 nm. The antimicrobial activity of the prepared nanoparticles was tested against several pathogenic bacteria and fungi. The obtained results proved that the nanoparticles succeeded to affect all the tested microbes in the following order: Bacillus cereus ATCC6633>Pseudomonas aeruginosa ATCC9027= Klebsiella pneumoniae ATCC13883>Vibrio cholera ATCC700=Candida albicans ATCC 700=Escherichia coli NCTC10418>Staphylococcus aureus ATCC6538. The minimum inhibitory concentration (MIC) of the prepared nanoparticles varied among the tested microbes at range of 12 mg/ml and 25 mg/ml. These results encourage the application of prepared TiO₂-Ag nanoparticles for treatment of microbe-contaminated waters.

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