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Effect of synthesis variables of plasma synthesized polymers on growth of HepG2 cells

Elizabeth PÉREZ-TEJADA1,4*, Juan MORALES-CORONA3, Luis Ernesto GÓMEZ-QUIRÓZ2, María Concepción GUTIERREZ-RUIZ2, Roberto OLAYO3

1 Departamento de Química. Universidad Autónoma Metropolitana, Unidad Iztapalapa, Apdo. Postal 55-534, Iztapalapa, CDMX, CP 09340
2 Departamento de Ciencias de la Salud. Universidad Autónoma Metropolitana, Unidad Iztapalapa, Apdo. Postal 55-534, Iztapalapa, CDMX, CP 09340
3 Departamento de Física. Universidad Autónoma Metropolitana, Unidad Iztapalapa, Apdo. Postal 55-534, Iztapalapa, México, D.F., CP 09340
4 CECyT No. 8, Instituto Politécnico Nacional, Ave. de las Granjas 618, Jardín Azpeitia, Azcapotzalco, CDMX, CP 02530

* Corresponding Author:* Addresss correspondence to: Elizabeth PÉREZ-TEJADA, email

BIOCELL 2017, 41(2-3), 41-44. https://doi.org/10.32604/biocell.2017.41.041

Abstract

Low pressure plasma polymer films were synthesized using pyrrole and allylamine monomers and adding iodine was used (or not) for the reaction in both cases. They were polymerized on glass substrates under the same reaction conditions. Polymerization of allylamine was also studied at different operating powers. These thin polymer films were used as culture surfaces for HepG2 cells, a cell line derived from a human hepatoma. The proliferation, differentiation and two-dimensional propagation until obtaining monolayer of the cells was studied on the different synthetized films and correlations were established between the conditions of synthesis, the physicochemical characteristics obtained and the performance as substrates for the cellular growth.

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PÉREZ-TEJADA, E., MORALES-CORONA, J., GÓMEZ-QUIRÓZ, L. E., GUTIERREZ-RUIZ, M. C., OLAYO, R. (2017). Effect of synthesis variables of plasma synthesized polymers on growth of HepG2 cells. BIOCELL, 41(2-3), 41–44.

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cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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