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ARTICLE
Time course and mechanism of brain oxidative stress and damage for redox active and inactive transition metals overload
Nidia FERRAROTTI1, Rosario MUSACCO-SEBIO2, Christian SAPORITO-MAGRIÑÁ2, Juan Manuel ACOSTA2, Marisa REPETTO2 *
1
Clinical Biochemistry Department, Faculty of Pharmacy and Biochemistry, Institute of Biochemistry and Molecular Medicine (IBIMOL),
University of Buenos Aires -CONICET, Buenos Aires, Argentina.
2
General and Inorganic Chemistry, Faculty of Pharmacy and Biochemistry, Institute of Biochemistry and Molecular Medicine (IBIMOL),
University of Buenos Aires -CONICET, Buenos Aires, Argentina.
* Address correspondence to: Marisa Repetto,
BIOCELL 2016, 40(1), 19-22. https://doi.org/10.32604/biocell.2016.40.019
Abstract
The objective of this work was to study the in vivo time course of biochemical processes of oxidative
damage in the brain of Sprague-Dawley rats that received an acute overload of the redox active metals iron (Fe)
and copper (Cu), and the redox inactive cobalt (Co) and nickel (Ni). Oxidative stress indicators (phospholipid and
protein oxidation), glutathione (GSH), antioxidant enzymes and NADPH oxidase activities, and the plasma inflammatory cytokine (IL-6) were measured. The results showed that in brain oxidative mechanisms for both sets of metal
are different, however in both cases are irreversible. The mechanism for Fe and Cu oxidative damage is mediated
by the generation of the free radical hydroxyl (Fenton reaction and homolytic cleavage of hydroperoxides). Two
events of antioxidant protection prior to oxidation of phospholipids and proteins by Fe and Cu are considered. The
first process is the use of GSH and the second is the increased activity of the Cu, Zn-SOD and catalase enzymes.
The oxidative mechanism for metal redox inactive is the consumption of GSH, NADPH oxidase activation and
inflammatory response mediated by IL-6. Co increased protein oxidation as a result of the inflammatory process. Ni
produced increments of phospholipid oxidation and SOD activity
Keywords
Cite This Article
FERRAROTTI, N., MUSACCO-SEBIO, R., SAPORITO-MAGRIÑÁ, C., ACOSTA, J. M., REPETTO, M. (2016). Time course and mechanism of brain oxidative stress and damage for redox active and inactive transition metals overload.
BIOCELL, 40(1), 19–22.