Open Access
ARTICLE
Ketamine effect on intracellular and mitochondrial calcium mobilization
Juanita BUSTAMANTE1,*, Analía CZERNICZYNIEC2, Silvia LORES-ARNAIZ2
1
Centro de Altos Estudios en Ciencias Humanas y de la Salud (CAECIHS), Facultad de Medicina, Universidad Abierta Interamericana,
Buenos Aires, Argentina
2
Instituto de Bioquímica y Medicina Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina
* Address correspondence to: Juanita Bustamante,
BIOCELL 2016, 40(1), 11-14. https://doi.org/10.32604/biocell.2016.40.011
Abstract
The suppressive effects of ketamine on intracellular calcium has been reported in a variety of cells
although the mechanisms involved are not well understood. The aim of this work was to evaluate the ketamine
effect on the mitochondrial Ca
2+ accumulation and the cellular Ca
2+ mobilization using FLUO4-AM and flow
cytometry. The results showed that mitochondria from ketamine injected animals presented a lower ability to
retain calcium at concentrations higher than 20 μM, as compared with controls (saline injected animals). In addition, ketamine showed a significant decreased KCl-induced intracellular calcium concentration. KCl increased
calcium influx through cellular depolarization. According to the data presented herein, ketamine presents a
clear inhibitory effect on cytosolic Ca
2+ transport mechanisms, independently from their action on the calcium
channel associated NMDA receptor.
Keywords
Cite This Article
BUSTAMANTE, J., CZERNICZYNIEC, A., LORES-ARNAIZ, S. (2016). Ketamine effect on intracellular and mitochondrial calcium mobilization.
BIOCELL, 40(1), 11–14. https://doi.org/10.32604/biocell.2016.40.011
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