Open Access
ARTICLE
Adenosine A1 receptor activation inhibits histamine release in gastric enterochromaffin-like cells
Rafael Martins de OLIVEIRA, Carolina Bernardi STEFANI, Angélica Aparecida Antoniellis SILVEIRA, Marcelo Lima RIBEIRO, José PEDRAZZOLI Jr., Alessandra GAMBERO*
Clinical Pharmacology and Gastroenterology Unit, São Francisco University Medical School, Bragança Paulista, SP, Brazil.
* Address correspondence to:Alessandra Gambero,
BIOCELL 2015, 39(2-3), 5-8. https://doi.org/10.32604/biocell.2015.39.005
Abstract
Adenosine acts as a gastroprotective factor decreasing inflammation and reducing gastric
acid secretion. Quantitative RT-PCR was used to determine the expression of the adenosine receptor genes
(A1AR, A2AAR, A2BAR and A3AR) and that of the gastrin receptor B gene
(CCKBR) in isolated, short-term
cultured enterochromaffin-like (ECL) cells. Both the A1AR and the
CCKBR genes were expressed at a level higher than the other genes. Also, the effect of 2-chloroadenosine, a stable agonist of A1 and A2A receptors, was
explored on ECL cells, with a resulting inhibition of both basal and gastrin-stimulated histamine release. Also,
dipropylcyclopentylxanthine (DPCPX), a selective A1 antagonist, prevented the inhibitory effects of 2-chloroadenosine, suggesting the effect of 2-chloroadenosine is mediated by A1 receptors. It is concluded that isolated,
short-term cultured ECL cells are a suitable model for studies relating gene expression and function, and that the
gastroprotective actions of adenosine are at least partly mediated through A1 receptors.
Keywords
Cite This Article
Martins, R., STEFANI, C. B., Aparecida, A., RIBEIRO, M. L., Jr., J. P. et al. (2015). Adenosine A1 receptor activation inhibits histamine release in gastric enterochromaffin-like cells.
BIOCELL, 39(2-3), 5–8. https://doi.org/10.32604/biocell.2015.39.005