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ARTICLE
Meiotic behavior and pollinic viability in bean cultivars
Gracieli DALLA NORA, Solange Bosio TEDESCO, Tamara PASTORI, Viviane Dal-Souto FRESCURA*, Nerinéia Dalfollo RIBEIRO, Haywood Dail LAUGHINGHOUSE IV, Sidinei José LOPES, Maria Teresa SCHIFINO-WITTMANN
Laboratory of Cytogenetics and Plant Genotoxicity, Department of Biology, Center for Natural and Exact Sciences, Federal University of Santa Maria (UFSM), Rio Grande do Sul, Brazil
* Address correspondence to: Viviane Dal-Souto Frescura,
BIOCELL 2014, 38(1), 33-38. https://doi.org/10.32604/biocell.2014.38.033
Abstract
The aims of this study were to determine the meiotic behavior and to estimate pollen grains viability in bean (Phaseolus vulgaris L.) cultivars. Flower buds were collected during different developmental stages
of the Mesoamerican bean cultivars IAPAR 44, Guapo Brilhante, BRS Expedito, BRS Valente, Guateian 6662
and Pérola, and the Andean bean cultivar Iraí, grown in a greenhouse. The meiotic index was determined by
anther squashing of material fixed in absolute ethanol-glacial acetic acid (3:1) and stained with acetic orcein.
No meiotic abnormalities were observed and the meiotic indices were high for all cultivars, indicating that the
mismatch generated during crosses is not related to any meiotic changes. Estimation of pollen viability was made
by comparing acetic orcein staining vs. Alexander’s reactive: pollen viability was high in all cultivars with either
stain, but was significantly higher when using the acetic orcein stain (>99%). Though some cultivar showed a
significantly smaller pollen size, the range of variation among cultivars was low (means’ range 51-66 μm)
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Cite This Article
APA Style
NORA, G.D., TEDESCO, S.B., PASTORI, T., FRESCURA, V.D., RIBEIRO, N.D. et al. (2014). Meiotic behavior and pollinic viability in bean cultivars . BIOCELL, 38(1), 33-38. https://doi.org/10.32604/biocell.2014.38.033
Vancouver Style
NORA GD, TEDESCO SB, PASTORI T, FRESCURA VD, RIBEIRO ND, IV HDL, et al. Meiotic behavior and pollinic viability in bean cultivars . BIOCELL . 2014;38(1):33-38 https://doi.org/10.32604/biocell.2014.38.033
IEEE Style
G.D. NORA et al., "Meiotic behavior and pollinic viability in bean cultivars ," BIOCELL , vol. 38, no. 1, pp. 33-38. 2014. https://doi.org/10.32604/biocell.2014.38.033
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