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Home / Journals / BIOCELL / Vol.37, No.3, 2013
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  • Open AccessOpen Access

    ARTICLE

    A likely role for the PH-domain containing protein, PEPP2/ PLEKHA5, at the membrane-microtubule cytoskeleton interface

    Yi ZOU1*, Timothy C COX2
    BIOCELL, Vol.37, No.3, pp. 55-61, 2013, DOI:10.32604/biocell.2013.37.055
    Abstract PH (pleckstrin homology) domains are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular compartments with assistances of alternative binding partners. PH domain-containing proteins have been found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2 (also known as PLEKHA5), displays moderate phosphoinositide binding specificity. Full length PEPP2 was observed to variably associate with both the plasma membrane and microtubules. The membrane-associated PEPP2 nucleated at cell-cell contacts and the More >

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  • Open AccessOpen Access

    ARTICLE

    Immune strategies of phagoctytic cells stimulated in vitro with live and heat-inactivated Streptococcus pyogenes

    Agustín César MÖHLINGERa, Virginia Paula SIFFREDIa, Serenela CHULIBERTa, Adriana PONESSAb, Rodolfo David NOTARIOb, Diana Graciela DLUGOVITZKYa
    BIOCELL, Vol.37, No.3, pp. 63-69, 2013, DOI:10.32604/biocell.2013.37.063
    Abstract Streptococcus pyogenes (group A Streptococcus) is frequently involved in a wide range of human diseases. Here we evaluated polymorphonuclear neutrophils and mononuclear cells from healthy subjects for their bactericidal function after stimulation with live and inactivated Streptococcus pyogenes (Streptococcus Group A). Mononuclear cells and Neutrophils were isolated from heparinized blood samples (n=18) using a Ficoll-Hypaque gradient and cultured in RPMI 1640 for 18 hours with a suspension of either live or inactivated Streptococcus pyogenes. Both the respiratory burst (flow cytometry) and nitrite, TNF and IL17 production (ELISA) were measured in the cell culture supernatants. An increased respiratory burst More >

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  • Open AccessOpen Access

    ARTICLE

    Angiotensin II stimulates expression of transcription factors c-Jun and c-Fos in cyclosporine induced human gingival fibroblasts

    Tamilselvan SUBRAMANI1,2, Suresh RAO2, Kamatchiammal SENTHILKUMAR3, Soundararajan PERIASAMY4, Noorjahan Banu ALITHEEN1
    BIOCELL, Vol.37, No.3, pp. 71-76, 2013, DOI:10.32604/biocell.2013.37.071
    Abstract The present study demonstrates that the expression of c-Jun and c-Fos are elevated in gingival fibroblast cells treated with angiotensin II and cyclosporine. The healthy human gingival tissues were collected and gingival fibroblasts were isolated and cultured. We used RT-PCR and Western blot analysis to identify the expression of c-Jun and c-Fos in cyclosporine and angiotensin II treated human gingival fibroblast cells. We found that angiotensin II in combination with cyclosporine induces c-Jun and c-Fos expressions significantly; however, the angiotensin II antagonist losartan inhibits the expression of c-Jun and c-Fos (p < 0.01). The data More >

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  • Open AccessOpen Access

    ARTICLE

    Exocytosis determination of SH-SY5Y single cell stimulated by different stimulants on indium tin oxide (ITO) micro-pore electrode

    Hui ZHAO1,2, Fan ZHANG1*, Ling LI1, Pingang HE1, Yuzhi FANG1
    BIOCELL, Vol.37, No.3, pp. 77-83, 2013, DOI:10.32604/biocell.2013.37.077
    Abstract The human neuroblastoma SH-SY5Y cell line has been used as a model to study mechanisms of neurotransmitter release. In order to study the mechanism of SH-SY5Y single cell exocytosis stimulated by different stimulants, including high K+, 3-(1-nitroso-2-pyrrolidinyl) pyridine and nicotine, a type of indium tin oxide (ITO) micro-pore electrode was used to obtain the corresponding amperometric response time. When the cell is stimulated by 0.1 M K+ , almost immediate exocytosis could be detected, due to the rapid depolarization of cell membrane. However, the stimulations with 1 mM nicotine and 3-(1-nitroso-2-pyrrolidinyl) pyridine result in a More >

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  • Open AccessOpen Access

    ARTICLE

    Ultrastructure of multicellular dwarf males with external gametangium in Oedogonium macrandrium (Oedogoniales, Chlorophyta)

    Silvana L. D’AMICO1, Patricia I. LEONARDI 1,2*, Eduardo J. CÁCERES1
    BIOCELL, Vol.37, No.3, pp. 85-90, 2013, DOI:10.32604/biocell.2013.37.085
    Abstract This is the first comprehensive ultrastructural study on dwarf males with external gametangia in the ge in the genus Oedogonium, from androspore germination to the liberation of mature male gametes. The ultrastructure of the process in O. macrandrium Wittrok is similar to that of Bulbochaete hiloensis (Nordstedt) Tiffany, butwith two remarkable differences. In O. macrandrium : 1) instead of a true transverse wall, only condensed mucilage appears between the gametes of each antheridial cell, and 2) the cell wall between the basal cell and the basal most antheridial cell has simple plasmodesmata similar to those More >

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