Open Access

ARTICLE

High passage numbers induce resistance to apoptosis in C2C12 muscle cells

by LUCIA PRONSATO, ANABELA LA COLLA, ANA CAROLINA RONDA, LORENA MILANESI, RICARDO BOLAND, ANDREA VASCONSUELO

Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur. San Juan 670, 8000 Bahía Blanca, Argentina

* Address correspondence to: Andrea Vasconsuelo. Departamento de Biología, Bioquímica y Farmacia. Universidad Nacional del Sur. San Juan 670, 8000 Bahía Blanca. Argentina. E-mail: Received: September 17, 2012.

BIOCELL 2013, 37(1), 1-9. https://doi.org/10.32604/biocell.2013.37.001

Abstract

Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2 O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2 O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2 O2 -induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.

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