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CHAOJUN TANG¹, XUE WU¹, LINQI YE^1,2, XIANG XIE¹, GUIXUE WANG^1*
BIOCELL, Vol.36, No.3, pp. 97-104, 2012, DOI:10.32604/biocell.2012.36.097
Abstract Devices for the rotational culture of cells and the study of biological reactions have been widely applied in tissue engineering. However, there are few reports exploring the effects of rotational culture on cell morphology, nitric oxide (NO) production, and cell cycle of the endothelial cells from human umbilical vein on the stent surface. This study focuses on these parameters after the cells are seeded on the stents. Results showed that covering of stents by endothelial cells was improved by rotational culture. NO production decreased within 24 h in both rotational and static culture groups. In More >

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JOSÉ LUIS ARREDONDO-FIGUEROA¹, LAURA GEORGINA NÚÑEZ-GARCÍA², PALOMA ADRIANA HEREDIA-GUZMÁN³ AND JESÚS T. PONCE-PALAFOX⁴.
BIOCELL, Vol.36, No.3, pp. 105-111, 2012, DOI:10.32604/biocell.2012.36.105
Abstract Chirostoma jordani is a native annual species inhabiting lacustrine waters of the Central Mexico Plateau. It is widely distributed and is currently facing high environmental pressures. Five experiments were performed to study the reproductive performance of this species. Four of the experiments were conducted in 270-L indoor recirculation tanks. Two males and one female at the first stage of reproduction were included in each test. A photoperiod of 14 light hours and 10 dark hours was used. In a fifth experiment, 10 females and 15 males were kept in an outdoor 3,000-L recirculation tank under natural… More >

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ARTICLE

LI-NA XU^1#, BI-NAN LU^2#, MING-MING HU¹, YOU-WEI XU¹, XU HAN¹, YAN QI¹, JIN-YONG PENG^1,3*
BIOCELL, Vol.36, No.3, pp. 113-120, 2012, DOI:10.32604/biocell.2012.36.113
Abstract Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner. Morphology of apoptotic cells was studied with acridine orange/ ethidium bromide… More >

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ARTICLE

MIROSLAV VAŘECHA^1*, DANIELA PÁCLOVÁ², JIŘINA PROCHÁZKOVÁ², PAVEL MATULA¹, DUŠAN CMARKO³, AND MICHAL KOZUBEK¹
BIOCELL, Vol.36, No.3, pp. 121-126, 2012, DOI:10.32604/biocell.2012.36.121
Abstract Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing… More >

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YI ZOU AND WENPING ZHONG
BIOCELL, Vol.36, No.3, pp. 127-132, 2012, DOI:10.32604/biocell.2012.36.127
Abstract The published article titled “A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton” has been retracted from the BIOCELL, Vol. 36, Issue 3, 2012. Title: A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton Authors: Yi Zou and Wenping Zhong URL: http://150.109.118.215/uploads/attached/file/20190102/20190102065508_87612.pdf The article “A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton” (Biocell 36, 127-132, 2012) has been retracted after publication by decision of the Editor-in-Chief, after he received compelling evidence indicating that the article’s content… More >

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ARTICLE

RANDI ZUKAS¹, ALEX J. CHANG¹, MARIAN RICE², AMY L. SPRINGER^1*
BIOCELL, Vol.36, No.3, pp. 133-142, 2012, DOI:10.32604/biocell.2012.36.133
Abstract Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. These motors are found in the inner arm and outer arm complexes. We are studying one of the inner arm dynein motors in the T. brucei flagellum: dynein-f. RNAi knockdown of genes for two components of dynein-f: DNAH10, the α heavy chain,… More >

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VIVIANE DAL-SOUTO FRESCURA¹, HAYWOOD DAIL LAUGHINGHOUSE IV², THAIS SCOTTI DO CANTODOROW¹, SOLANGE BOSIO TEDESCO^1*
BIOCELL, Vol.36, No.3, pp. 143-145, 2012, DOI:10.32604/biocell.2012.36.143
Abstract Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as ‘barba-de-São-João’, ‘barba-de-bode’, ‘vassourinha branca’, and ‘mimosa’. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander’s stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) More >

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