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ARTICLE
Micrometric measurement of the density of stained odontoblast processes
ALICIA KOHLI1 , STELLA M. PEZZOTTO2 , GRACIELA GARCIA3 , LEONOR C. POLETTO2.
1. Histology and Embryology Department, School of Dentistry, National University of Rosario. Argentina.
2. Research Council, National University of Rosario. Argentina.
3. Biochemistry School. National University of Rosario. Argentina.
*Address correspondence to: Alicia Kohli. Pasaje Rizzuto 3473, (2000) Rosario, Argentina. E-mail:
BIOCELL 2011, 35(2), 51-58. https://doi.org/10.32604/biocell.2011.35.051
Abstract
The embryological, structural and functional unit of the dentine-pulp complex shares the odontoblast, located in the border of the dentine pulp, with basal nuclei and organelles. The odontoblast process
emerges from its apical pole. It is formed by microtubules, microfilaments and vesicles covered by membranes penetrating the dentinal tubules, isolated from the inter-tubular matrix, along the extent of the dentine.
The objective of this study was to evaluate the efficacy of three staining techniques: hematoxylin-eosin,
periodic acid-Schiff and Schmorl, by staining the process, from beginning to end, and compare the results
with the erosion technique. Thirty human teeth were employed in the trial; after their extraction the pulp was
fixated, the pieces demineralized in nitric acid at 8%, the collagen filaments eliminated with Type II Collagenase, the tissue was stained, and the measurements were made. The portions with no pulp were prepared with
the erosion technique.
Results: Comparing the best results obtained by staining with the values obtained with the erosion technique,
the former showed lower values. Conclusion: Staining techniques show lower density of the staining processes compared with the dentinal tubules in the erosion technique.
Keywords
Cite This Article
KOHLI, A. (2011). Micrometric measurement of the density of stained odontoblast processes.
BIOCELL, 35(2), 51–58. https://doi.org/10.32604/biocell.2011.35.051