TY - EJOU
AU - SALAMONE, ROMINA J. BEVACQUA, RAFAEL FERNANDEZ-MARTIN, DANIEL F.
TI - Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
T2 - BIOCELL
PY - 2011
VL - 35
IS - 1
SN - 1667-5746
AB - Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human
embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos
inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the
second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to
block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the
diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes (77%) were arrested at a meiotic stage characterized by the presence of a
large internal metaphase plate and absence of polar body. In experiment 2, development of embryos exposed
to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared.
Developmental rates did not differ between diploidization strategies, even when three agents were employed
to induce activation. Both groups, CytoB-IVM and CytoB-ACT, tended to maintain diploidy. CytoB-IVM
parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor.
KW - meiotic maturation
KW - microfilaments
KW - cytochalasin B
KW - parthenogenesis
DO - 10.32604/biocell.2011.35.001