BIOCELL-Vol. 35, No. 1, 2011

Open Access

ARTICLE

Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
ROMINA J. BEVACQUA, RAFAEL FERNANDEZ-MARTIN, DANIEL F. SALAMONE
BIOCELL, Vol.35, No.1, pp. 1-7, 2011, DOI:10.32604/biocell.2011.35.001
Abstract Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid… More >

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ARTICLE

Salinity induced anatomical and morphological changes in Chloris gayana Kunth roots
GABRIEL CÉCCOLI^*1, JULIO C. RAMOS¹, LEANDRO I. ORTEGA² , JUAN M. ACOSTA¹, MARIEL G. PERRETA¹
BIOCELL, Vol.35, No.1, pp. 9-17, 2011, DOI:10.32604/biocell.2011.35.009
Abstract Chloris gayana Kunth is a grass species valuable as forage which was introduced into Argentina to be used as pasture in saline soils of subtropical and warm-temperate zones, given its good adaptability to drought, salinity and mild freezing. However, its tolerance varies according to the cultivar. In tetraploid cultivars, important reductions in yield have been observed. Here, a study of the variations produced on the root and stem system by salinity at different NaCl concentrations (0, 150 y 250 mM) was performed in the Boma cultivar, with the aim of determining the anatomical and morphological alterations More >

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ARTICLE

Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
PAOLA TALIA¹ , EDUARDO J. GREIZERSTEIN¹ , H. ESTEBAN HOPP^1,2 , NORMA PANIEGO¹ , LIDIA POGGIO2 AND RUTH A. HEINZ^1,2*
BIOCELL, Vol.35, No.1, pp. 19-28, 2011, DOI:10.32604/biocell.2011.35.019
Abstract Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG More >

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Evaluation of potential embryo toxicity of albendazole sulphoxide in CF1 mice
MIRIAM TERUEL^1,2, JAQUELINE D’ERCOLE¹ AND RODOLFO CATALANO²
BIOCELL, Vol.35, No.1, pp. 29-34, 2011, DOI:10.32604/biocell.2011.35.029
Abstract Benzimidazole compounds are used in both humans and animals for controlling helminth parasites. Albendazole has teratogenic effects attributed to its active metabolite albendazole sulphoxide. The aim of this work was to evaluate the effect of the latter compound when administered to pregnant CF1 mice during the preimplantation period. Females were superovulated by intraperitoneal injection of 10 IU of eCG and 10 IU of hCG (48h later) and were paired with males of proven fertility. Albendazole sulphoxide (200 mg/kg) was orally administered by gavages at day 1, 2 or 3 of pregnancy; the control group received More >

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REVIEW

Brief Note: A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR
G. P. CARDOZO¹ , E. V. SANTOS¹ , A. L. FACHIN¹, S. C. FRANÇA¹ , AND M. MARINS^1,2,*
BIOCELL, Vol.35, No.1, pp. 35-36, 2011, DOI:10.32604/biocell.2011.35.035
Abstract E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR. More >

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