Open Access

ARTICLE

Inhibitory effect of jasmonic acid and ethylene on epicotyl growth and bud induction in the maritime pine, Pinus pinaster Soland. in Ait

MARIA TERESA MARTIN^2*, HILDA PEDRANZANI³, PATRICIA GARCÍA-MOLINERO², VALENTIN PANDO⁴, ROSARIO SIERRA-DE-GRADO¹

1. Departamento de Producción Vegetal y Recursos Forestales, Escuela Técnica Superior de Ingenierías Agrarias, Universidad de Valladolid, Avda. de Madrid, 44; Palencia E-34007 Spain.
2. Departamento de Viticultura, Instituto Tecnológico Agrario de Castilla y León, Ctra. Burgos, Km. 119; Valladolid E47071. ESPAÑA.
3. Laboratorio de Fisiología Vegetal, Departamento de Ciencias Agropecuarias. FICES Universidad Nacional de San Luis, Avda. 25 de Mayo 384, Villa Mercedes, San Luis, Argentina.
4. Departamento de Estadística e Investigación Operativa. Escuela Técnica Superior Ingenierías Agrarias. Universidad de Valladolid. Avda. de Madrid 44; Palencia E-34007 Spain.
* Address correspondence to: Maria Teresa Martin. E-mail: marvilte@itacyl.es

BIOCELL 2009, 33(3), 141-148. https://doi.org/10.32604/biocell.2009.33.141

Abstract

Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasmonic acid, 6-benzylaminopurine or Ethephon (which is converted to ethylene in plants) in any of treatments. Concentrations of 100 μM of jasmonic acid and Ethephon had a greater inhibitory effect than the treatments with 10 μM. In addition to that, jasmonic acid was a stronger inhibitor than Ethephon in any of the tried combinations. There were no significant differences between the control treatment and the treatments with only 10 μM of jasmonic acid or Ethephon. However, 10 μM 6-benzylaminopurine induced bud formation. The different combinations of 6-benzylaminopurine with jasmonic acid and Ethephon showed that concentrations of 10 to 100 μM did not affect the number of induced buds. Jasmonic acid had an inhibitory effect which Ethephon only showed when combined with 100 μM of jasmonic acid and 10 μM of 6-benzylaminopurine. Three response groups were defined by cluster analysis: group 1 produced the greatest mean number of buds (4 to 5) and a mean epicotyl growth of 1 to 1.5 cm; group 2 produced 2 to 4 buds and a mean growth of 0.5 to 1.2 cm; group 3 produced only one bud and a mean epicotyl length of 1.2 to 2 cm.

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