Open Access

ARTICLE

Isolation and molecular characterization of a cax gene from Capsella bursa-pastoris

by JUAN LIN, WEN ZHANG, MINGZHU SHI, XINGLONG WANG, XIAOFEN SUN, KEXUAN TANG

1. State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU- Nottingham Plant Biotechnology R&D Center, and Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai 200433, People’s Republic of China.
2. Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, People’s Republic of China.
Address correspondence to: Juan Lin. State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, CHINA. Fax: (+86 21) 65642425. E-mail: linjuan@fudan.edu.cn

BIOCELL 2008, 32(3), 229-235. https://doi.org/10.32604/biocell.2008.32.229

Abstract

A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursapastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na⁺/Ca²⁺ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated that Cbcax51 could be activated by cold triggering and was related to the cold acclimation process, but its expression is regulated negatively by drought and not affected by ABA or salt.

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