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MARTHA LAFARQUE, LILIANA OLIVEROS
BIOCELL, Vol.32, No.3, pp. 211-218, 2008, DOI:10.32604/biocell.2008.32.211
Abstract In this study, evidence for a factor secreted by bovine hypophyseal pars tuberalis that stimulates luteinizing hormone (LH) release from rat pars distalis cells is shown. The secretion products of bovine pars tuberalis cells into the culture medium were assayed on dispersed rat pars distalis cells in 30 min incubations and superfusion experiments. The culture medium from pars tuberalis total cell populations, added at a dose of 6 μg per tube, induced the greater LH release from pars distalis cells, without effect on follicle stimulating hormone (FSH) release. After pars tuberalis cells separation on a discontinuos Percoll gradient, only the culture medium… More >

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OSCAR MAC DONAL¹, JUAN G. CHEDIACK^1,2,3, ENRIQUE CAVIEDES-VIDAL^1,2,3
BIOCELL, Vol.32, No.3, pp. 219-227, 2008, DOI:10.32604/biocell.2008.32.219
Abstract The isolation of viable enterocytes, villi and crypts from the small intestine of a feral bird (Columba livia) is important for performing physiological experiments in ecologically relevant processes of membrane transport. The effectiveness of mechanical disruption, enzymatic digestion and chelating agents were compared. The objectives were to isolate enterocytes, villi and crypts from the small intestine of young pigeons; to evaluate the viability of the isolated intestinal epithelial cells isolated; and to verify the integrity of enterocytes by biochemical features. Enzymatic and mechanical methods yielded both elongated columnar and spherical cells. With the chelating method villi More >

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JUAN LIN¹, WEN ZHANG¹, MINGZHU SHI¹, XINGLONG WANG¹, XIAOFEN SUN¹, KEXUAN TANG^1,2
BIOCELL, Vol.32, No.3, pp. 229-235, 2008, DOI:10.32604/biocell.2008.32.229
Abstract A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursapastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na⁺/Ca²⁺ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments More >

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ELEONIDAS MOURA LIMA^1,2, MARIANA FERREIRA LEAL³, MARÍLIA DE ARRUDA CARDOSO SMITH³, ROMMEL RODRÍGUEZ BURBANO⁴, PAULO PIMENTEL DE ASSUMPÇÃO⁵, MARIA JOSE BELLO⁶, JUAN ANTONIO REY⁶, FRANCINALDO FERREIRA DE LIMA⁷, CACILDA CASARTELLI²
BIOCELL, Vol.32, No.3, pp. 237-243, 2008, DOI:10.32604/biocell.2008.32.237
Abstract Gastric cancer is one of the most common malignancies. DNA methylation is implicated in DNA mismatch repair genes deficiency. In the present study, we evaluated the methylation status of MLH1, MSH2, MSH6 and PMS2 in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosal of gastric cancer patients from Northern Brazil. We found that none of the nonneoplastic samples showed methylation of any gene promoter and 50% of gastric cancer samples showed at least one methylated gene promoter. Methylation frequencies of MLH1, MSH2, MSH6 and PMS2 promoter were 21.74%, 17.39%, 0% and 28.26% respectively… More >

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IIZUKA YOKO¹, SAMIN HONG², CHAN YUN KIM², SEUNG KAB KIM², GONG JE SEONG²
BIOCELL, Vol.32, No.3, pp. 245-250, 2008, DOI:10.32604/biocell.2008.32.245
Abstract Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine, agmatine (0.0 to 100.0 μM) pretreatment was performed for 2 hours. Subsequently, they were exposed to hydrogen peroxide (0.0 to 2.5 mM) as an oxidative stress. Cell viability was monitored for up to 48 hours with the lactate dehydrogenase (LDH) assay and apoptosis was examined by the terminal deoxynucleotide transferase-mediated terminal More >

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SILVIA S. BORKOSKY^1,2, HITOSHI NAGATSUKA¹, YORIHISA ORITA³, HIDETSUGU TSUJIGIWA⁴, JUNKO YOSHINOBU³, MEHMET GUNDUZ¹, ANDREA P. RODRIGUEZ¹, LILIANA R. MISSANA², KAZUNORI NISHIZAKI³, NORIYUKI NAGAI¹
BIOCELL, Vol.32, No.3, pp. 251-257, 2008, DOI:10.32604/biocell.2008.32.251
Abstract The Notch signaling pathway is an evolutionary conserved mechanism that plays an important role in cell-cell communication and cell fate in a wide range of tissues. The mammalian family of Notch receptors consists of 4 members: Notch1/2/3/4. The Notch ligand family consists of 5 members: Delta1/3/4 and Jagged1/2. Math1 encodes a murine basic helix-loop-helix (bHLH) transcription factor that acts as positive regulator of cell differentiation. Recently, links between Notch and Math1 pathways were demonstrated in various tissues. Expression of Notch1, Jagged2 and Math1 were analyzed in the mouse molar tooth germ during embryonic stage (E) 13 and E15… More >

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874

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JIANLI SHI, WENYAN MEI, JING YANG
BIOCELL, Vol.32, No.3, pp. 259-263, 2008, DOI:10.32604/biocell.2008.32.259
Abstract As the key component of many hemoproteins (heme-containing proteins), heme is involved in a broad range of biological processes. Enzymes required for heme biosynthesis and degradation pathways are evolutionarily conserved. While heme metabolism has been studied extensively, the expression of heme metabolism enzymes during development has not been described. Here, we report that all heme biosynthases and two heme oxygenases, which initiate heme degradation, are dynamically expressed during Xenopus embryonic development. All heme synthases, with the exception of aminolevulinic acid synthase 2, are maternally expressed. At neurula stage, heme synthases are expressed in the developing neural… More >

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