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Genotoxic effect of Physalis angulata L. (Solanaceae) extract on human lymphocytes treated in vitro

RAQUEL ALVES DOS SANTOS1, TERESINHA ROSA CABRAL2, ISABEL ROSA CABRAL2, LUSÂNIA MARIA GREGGI ANTUNES3,4, CRISTIANE PONTES ANDRADE3, PLÍNIO CERQUEIRA DOS SANTOS CARDOSO1, MARCELO DE OLIVEIRA BAHIA2, CLAUDIA PESSOA5, JOSÉ LUIS MARTINS DO NASCIMENTO2, ROMMEL RODRÍGUEZ BURBANO2, CATARINA SATIE TAKAHASHI1,6

1. Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
2. Centro de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
3. Departamento de Ciências Biológicas, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil.
4. Departamento Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
5. Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
6. Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Address correspondence to: Dr. Rommel Rodríguez Burbano. Centro de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, BRASIL. E-mail: rommel@ufpa.br

BIOCELL 2008, 32(2), 195-200. https://doi.org/10.32604/biocell.2008.32.195

Abstract

Physalis angulata L (Solanaceae) is a medicinal plant from North of Brazil, whose different extracts and infusions are commonly used in the popular medicine for the treatment of malaria, asthma, hepatitis, dermatitis and rheumatism. However, the genotoxic effects of P. angulata on human cells is not well known. The main purpose of the present study was to evaluate the in vitro genotoxic effects of aqueous extract of P. angulata using the comet assay and the micronucleus assay in human lymphocytes provided from 6 healthy donors. Treatments with P. angulata extracts were performed in vitro in order to access the extent of DNA damage. The comet assay has shown that treatments with P. angulata at 0.5, 1.0, 2.0, 3.0 and 6.0 μg/mL in culture medium were genotoxic. Lymphocytes treated with P. angulata at the concentrations of 3.0 and 6.0 μg/mL in culture medium showed a statistically significant increase in the frequency of micronucleus (p<0.05), however, the cytokinesis blocked proliferation index (CBPI) was not decreased after P. angulata treatment. In conclusion, the present work demonstrated the genotoxic effects of P. angulata extract on human lymphocytes in vitro.

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APA Style
SANTOS, R.A.D., CABRAL, T.R., CABRAL, I.R., ANTUNES, L.M.G., ANDRADE, C.P. et al. (2008). Genotoxic effect of physalis angulata L. (solanaceae) extract on human lymphocytes treated in vitro. BIOCELL, 32(2), 195-200. https://doi.org/10.32604/biocell.2008.32.195
Vancouver Style
SANTOS RAD, CABRAL TR, CABRAL IR, ANTUNES LMG, ANDRADE CP, CARDOSO PCDS, et al. Genotoxic effect of physalis angulata L. (solanaceae) extract on human lymphocytes treated in vitro. BIOCELL . 2008;32(2):195-200 https://doi.org/10.32604/biocell.2008.32.195
IEEE Style
R.A.D. SANTOS et al., “Genotoxic effect of Physalis angulata L. (Solanaceae) extract on human lymphocytes treated in vitro,” BIOCELL , vol. 32, no. 2, pp. 195-200, 2008. https://doi.org/10.32604/biocell.2008.32.195

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cc Copyright © 2008 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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