Open Access

ARTICLE

Ultrastructural analysis and identification of membrane proteins in the free-living amoeba Difflugia corona

MARCELO SILVA-BRIANO¹ , SANDRA LUZ MARTÍNEZ-HERNÁNDEZ¹ , ARACELI ADABACHE-ORTÍZ¹ , JAVIER VENTURA-JUÁREZ² , EVA SALINAS³ , J. LUIS QUINTANAR⁴

1 Dept. of Biology,
2 Dept. Morphology,
3 Dept. de Microbiology and
4 Dept. of Physiology and Pharmacology. Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes. Av. Universidad 940 C.P. 20100. Aguascalientes Ags. México.
Address correspondence to: Dr. J. Ventura-Juárez. Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes. Av. Universidad 940. CP. 20100, Aguascalientes Ags., MEXICO. Fax: 0052-449-9108401. E-mail: jventur@correo.uaa.mx and venturajj@yahoo.com

BIOCELL 2007, 31(2), 225-228. https://doi.org/10.32604/biocell.2007.31.225

Abstract

Syntaxin-1 and 25-kDa Synaptosome-associated Protein (SNAP-25) are present in the plasma membrane of several different secretory cell types and are involved in the exocytosis process. In this work, the free-living amoeba Difflugia corona was studied in relation to ultrastructure, structural membrane proteins, and proteins such as Syntaxin-1 and SNAP-25. Our results obtained by scanning electron microscopy in the amoeba without its theca, showed many membrane projections and several pore-like structures. Using immunocytochemistry, we found structural proteins Syntaxin-1 and SNAP-25.

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Citations