Open Access

ARTICLE

Behavior and preservation of an in vitro collection of European aspen in Spain

M.T. MARTIN^*, H.E. PEDRANZANI^**, R. SIERRA DE GRADO

Dpt. Producción Vegetal y Recursos Forestales, Escuela Técnica Superior Ingenierías Agrarias, Universidad de Valladolid, Avda. de Madrid, 57; Palencia E-34071 SPAIN.
* Current address: Dpt. Viticultura, Instituto Tecnológico Agrario de Castilla y León, Ctra. Burgos, Km. 119; Valladolid E47071 SPAIN. Tel: (34) 983 414427, Fax: (34) 983 414480, email marvilte@itacyl.es
** Laboratorio de Fisiología Vegetal, Departamento de Ciencias Agropecuarias. FICES Universidad Nacional de San Luis, Avda. 25 de Mayo 384, (5730) Villa Mercedes, San Luis, Argentina.
Address correspondence to: Dra. M.T. Martin. Dpt. Viticultura, Instituto Tecnológico Agrario de Castilla y León, Ctra. Burgos, Km. 119; Valladolid E-47071, SPAIN. Phone: (+34) 983 414427. Fax: (+34) 983 414480. E-mail: marvilte@itacyl.es

BIOCELL 2007, 31(1), 41-49. https://doi.org/10.32604/biocell.2007.31.041

Abstract

An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations.
Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting stem explants from each clone were cultured in parallel in the different media. One and three months after setting on the stem explants, the number and the size of shoots, the root size, the presence or absence of callus and the survival, were evaluated. The survival was monthly recorded during 8 months. Taking into account the explant development, four media were proposed for collection preservation. One of them, Ga, with a reduction of salts, sucrose, 6-benzoaminopurine, omitting adenine sulphate and 1-naphthalene acetic acid, is the most economical. Behavior observations of the 32 clones in the 15 medium compositions showed the influence of the genotype of the clones.

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