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ARTICLE

Brief Note : Plant regeneration after long term callus culture in clones of Asparagus officinalis L.*

by A. C. PONTAROLI, E. L. CAMADRO

Estación Experimental Agropecuaria (EEA) Balcarce, Instituto Nacional de Tecnología Agropecuaria (INTA) and Facultad de Ciencias Agrarias (FCA), Universidad Nacional de Mar del Plata (UNMdP); C. C. 276, (7620) Balcarce, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Current Address: Dept.of Genetics, University of Georgia, 30602 Athens, Georgia, U.S.A.
Address correspondence to: Ing. Agr. Elsa L. Camadro. Casilla de Correo 276, (7620) Balcarce, ARGENTINA. FAX: (+54-2266) 439101. E-mail: ecamadro@balcarce.inta.gov.ar

BIOCELL 2005, 29(3), 313-317. https://doi.org/10.32604/biocell.2005.29.313

Abstract

Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l^-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l^-1 2,4-D and 1 mg.l^-1 kinetin. Shoot primordia (SP) induction from >18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone ‘265’ on MS medium with 5 mg.l^-1 2iP, 1 mg.l^-1 IAA, 3% sucrose and 0.9% agar. The highest percentage of root induction (100%) was achieved with clone ‘265’ on MS medium with 0.1 mg.l^-1 kinetin, 0.1 mg.l^-1 NAA, 1.32 mg.l^-1 ancymidol, 7% glucose and 0.8% agar. Important medium x genotype interactions were detected, pointing to the need of adjusting this and other in vitro protocols for specific asparagus genotypes.

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