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NORA MOHAMAD, ALICIA GUTIÉRREZ, MARIEL NÚÑEZ, CLAUDIA COCCA, GABRIELA MARTÍN*, GRACIELA CRICCO, VANINA MEDINA, ELENA RIVERA, ROSA BERGOC*.
BIOCELL, Vol.29, No.2, pp. 149-161, 2005, DOI:10.32604/biocell.2005.29.149
Abstract Apoptosis or programmed cell death (PCD) is a physiological process characteristic of pluricellular organisms leading to self-destruction of the cell. It is therefore involved in development, homeostasis and host defense. However, a significant difference has been shown between mammalian cell apoptosis and non-mammalian cell apoptosis: mitochondria are implicated only in the former. Execution of PCD includes the release of several proapoptotic proteins from the intermembrane space of mitochondria. They could exert their actions through a caspase dependent as well as a caspase independent way. On the other hand, regulation of PCD is mainly given by More >

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HILDA L. MONTRULL, NILDA Y. BRIZUELA, SILVIA L. DEMURTAS, LUIS SPITALE* , CARLOS I. MEIROVICH.
BIOCELL, Vol.29, No.2, pp. 163-167, 2005, DOI:10.32604/biocell.2005.29.163
Abstract Cartilage samples were taken from OA patients in order to describe and quantify pro-inflammatory mediators. Samples were cultured under aseptic conditions in Dulbecco’s modified Eagle medium at 37°C for 10 days. Control samples, taken from non-inflammatory cartilage, were cultured under the same conditions. The levels of NO^-₂ and NO^-₃ were measured in the supernatant using a spectrophotometric assay. The activity of MMP-1 was quantified by ELISA.
The concentration of NO^-_x was 47.3 ± 4.1 µM in the OA cartilague and 10.7 ± 1.8 µM in the controls. The average MMP-1 activity was 3,650 ± 387 ng/ml More >

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A. DEL CAMPO*, M. BRACHO, L. MARCANO, J. GUÍÑEZ, C. DE LA TORRE**
BIOCELL, Vol.29, No.2, pp. 169-176, 2005, DOI:10.32604/biocell.2005.29.169
Abstract This work evaluated the qualitative and quantitative cellular changes induced by treatment with 5-aminouracil (5-AU) and a combination of 5-AU and caffeine in plant cells in relation to DNA damage, repaired damage, and residual damage. As biological material, Allium cepa L. root tips were used, grown in filtered water, in darkness, with aeration at constant temperature of 25 °C ± 0.5. Cell populations were synchronized using 5 mM caffeine in order to study the effects of 5-AU and caffeine/5-AU combined treatment on the DNA content and their incidence in the entrance to mitosis. The results showed More >

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NILTON CESAR PIRES BIONE¹, MARIA SUELY PAGLIARINI², LEONES ALVES DE ALMEIDA³
BIOCELL, Vol.29, No.2, pp. 177-181, 2005, DOI:10.32604/biocell.2005.29.177
Abstract A spontaneous male-sterile, female-fertile mutation affecting bivalent arrangement at the metaphase plate and cytokinesis was detected in line BR98-197 of the soybean breeding program developed by Embrapa – National Soybean Research Centre. Untill diakinesis, meiosis was normal with chromosome pairing as bivalents. From this phase, in several meiocytes, bivalents were not able to organize a single metaphase plate and remained scattered in the cytoplasm in a few or several groups. In these meiocytes, chromosomes segregated in both divisions giving rise to several micronuclei. However, the main cause of male sterility was the absence of cytokinesis More >

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M.T. TERUEL^1,2, R.C. CATALANO², J.A. CABODEVILA², S.S. CALLEJAS²
BIOCELL, Vol.29, No.2, pp. 183-186, 2005, DOI:10.32604/biocell.2005.29.183
Abstract Embryo development depends on maternal and embryonic factors. When occurs in vitro, embryos secrete factors that stimulate their development. The purpose of this study was to investigate the possible effects of embryos at morula stage on mouse embryo development in vitro. To obtain conditioned media (CM), morulas were cultured in groups of 5 (CM5) or 10 (CM10) in microdrops of Ham-F10 culture medium during 24 h and later they were removed. Subsequently, 365 morulas were cultured in CM5 and CM10 or in Ham-F10 media (as control group). No differences in blastocyst formation could be found between embryos… More >

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ZHONGHAI CHEN¹, YONGZHEN PANG¹, XIAOJUN LIU¹, XINGLONG WANG¹, ZHONGXIANG DENG¹, XIAOFEN SUN¹, KEXUAN TANG^1,2
BIOCELL, Vol.29, No.2, pp. 187-193, 2005, DOI:10.32604/biocell.2005.29.187
Abstract Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (ZAA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA More >

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M.I. PIGOZZI, R.B. SCIURANO, A.J. SOLARI
BIOCELL, Vol.29, No.2, pp. 195-203, 2005, DOI:10.32604/biocell.2005.29.195
Abstract A testicular biopsy from an infertile man carrying a heterozygous chromosome translocation t(11;14) was studied with synaptonemal complex analysis and immunolocalization of the protein MLH1 for crossover detection. A full blockage of spermatogenesis at the spermatocyte stage was related to the presence of the translocation quadrivalents at pachytene. Only 2% of the quadrivalents showed full synapsis. Most of the spermatocytes showed asynaptic free ends that frequently mingled with the XY pair. The average number of crossovers per cell was diminished from a mean of 52.7 in controls to a mean of 48 in the patient. More >

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JOEL ARNEODO*, SOLEDAD DE BREUIL*, SERGIO LENARDON**, LUIS CONCI**
BIOCELL, Vol.29, No.2, pp. 205-207, 2005, DOI:10.32604/biocell.2005.29.205
Abstract Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture - reverse transcription - polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina. More >

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G.C. DALVIT, P.D. CETICA, L.N. PINTOS, M.T. BECONI
BIOCELL, Vol.29, No.2, pp. 209-212, 2005, DOI:10.32604/biocell.2005.29.209
Abstract Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO₂: 95% humidified air. In vitro fertilization was carried out in… More >

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