Open Access

ARTICLE

Influence of genotype and explant source on indirect organogenesis by in vitro culture of leaves of Melia azedarach L.

by S.K. VILA*, H.Y. REY, L.A. MROGINSKI

Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE). C.C. 209. (3400) Corrientes, Argentina.
Address correspondence to: Ing. Agr. Silvia K. Vila. Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias (UNNE). C.C. 209, (3400) Corrientes, ARGENTINA. Fax: (+54-3783) 427131; E-mail: ibone@agr.unne.edu.ar

BIOCELL 2004, 28(1), 35-41. https://doi.org/10.32604/biocell.2004.28.035

Abstract

In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10 – 15 years old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) supplemented with 1mg.l^-1 benzylaminopurine (BAP) + 0.1mg.l^-1 kinetin (KIN) + 3 mg.l^-1 adenine sulphate (ADS).
The rachis of the leaflets of the completely expanded leaves was found to be the most responsive tissue, in most of the genotypes employed. Shoot regeneration occurred in leaf explants of all the genotypes tested. The best genotype for shoot regeneration was clone 4. Rooting was induced on MS medium supplemented with 2.5 mg.l^-1 3-indolebutyric acid, IBA, (4 days) followed by subculture on MS lacking growth regulators (26 days). Complete plants were transferred to soil.

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