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ARTICLE
Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment
MARIANA N. MELCHIORRE, HERNÁN R. LASCANO, VICTORIO S.TRIPPI
Instituto de Fitopatología y Fisiología Vegetal. (IFFIVE-INTA). Camino 60 Cuadras Km 5 ˚. (X5020ICA) Córdoba, Argentina.
Corresponding Author: Mariana N. Melchiorre, Fax: (+54-351) 497 4340; E-mail:
BIOCELL 2002, 26(2), 217-223. https://doi.org/10.32604/biocell.2002.26.217
Abstract
Wheat (
Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embriogenic calli generation from inmature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene β-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by β-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which “model” cultivar was used.
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APA Style
MELCHIORRE, M.N., LASCANO, H.R., S.TRIPPI, V. (2002). Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment. BIOCELL, 26(2), 217-223. https://doi.org/10.32604/biocell.2002.26.217
Vancouver Style
MELCHIORRE MN, LASCANO HR, S.TRIPPI V. Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment. BIOCELL . 2002;26(2):217-223 https://doi.org/10.32604/biocell.2002.26.217
IEEE Style
M.N. MELCHIORRE, H.R. LASCANO, and V. S.TRIPPI "Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment," BIOCELL , vol. 26, no. 2, pp. 217-223. 2002. https://doi.org/10.32604/biocell.2002.26.217
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