Open Access

ARTICLE

Research progress and prospects of nucleic acid isothermal amplification technology

by SHUHUI WU^1,2,#, PING XU^3,#, XIANGBIN XU², SONG-BAI LIU^1,2,*

1 Suzhou Key Laboratory of Medical Biotechnology, Suzhou Vocational Health College, Suzhou, 215009, China
2 School of Food Science and Engineering, Hainan University, Haikou, 570228, China
3 The Affiliated Infectious Diseases Hospital, Suzhou Medical College of Soochow University, Suzhou, 215100, China

* Corresponding Author: Song-Bai Liu,
# Co-first authors

(This article belongs to the Special Issue: Bioinformatics Study of Diseases)

BIOCELL 2023, 47(11), 2385-2395. https://doi.org/10.32604/biocell.2023.029687

Received 03 March 2023; Accepted 19 April 2023; Issue published 27 November 2023

Abstract

Nucleic acid (DNA and RNA) detection and quantification methods play vital roles in molecular biology. With the development of molecular biology, isothermal amplification of DNA/RNA, as a new molecular biology technology, can be amplified under isothermal condition, it has the advantages of high sensitivity, high specificity, and high efficiency, and has been applied in various fields of biotechnology, including disease diagnosis, pathogen detection, food hygiene and safety detection and so on. This paper introduces the progress of isothermal amplification technology, including rolling circle amplification (RCA), nucleic acid sequence-dependent amplification (NASBA), strand displacement amplification (SDA), loop-mediated isothermal amplification (LAMP), helicase-dependent amplification (HDA), recombinase polymerase amplification (RPA), cross-priming amplification (CPA), and its principle, advantages and disadvantages, and application development are briefly summarized.

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Keywords

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