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  • Open Access

    ARTICLE

    Knockdown of circular RNA (CircRNA)_001896 inhibits cervical cancer proliferation and stemness in vivo and in vitro

    JIA SHAO1,2, CAN ZHANG2, YAONAN TANG2, AIQIN HE2, WEIPEI ZHU1,*
    BIOCELL, DOI:10.32604/biocell.2024.049092
    (This article belongs to this Special Issue: MicroRNA as Biomarkers for Disease Diagnosis and Progression)
    Abstract Objective:: Previous studies indicated that aberrant circular RNA (circRNA) expression affects gene expression regulatory networks, leading to the aberrant activation of tumor pathways and promoting tumor cell growth. However, the expression, clinical significance, and effects on cell propagation, invasion, and dissemination of circRNA_001896 in cervical cancer (CC) tissues remain unclear. Methods:: The Gene Expression Omnibus (GEO) datasets (GSE113696 and GSE102686) were used to examine differential circRNA expression in CC and adjacent tissues. The expression of circRNA_001896 was detected in 72 CC patients using fluorescence quantitative PCR. Correlation analysis with clinical pathological features was performed through COX multivariate and univariate analysis.… More >

  • Open Access

    REVIEW

    Do tensile and shear forces exerted on cells influence mechanotransduction through stored energy considerations?

    FREDERICK H. SILVER1,2,*, TANMAY DESHMUKH2
    BIOCELL, DOI:10.32604/biocell.2024.047965
    Abstract All tissues in the body are subjected externally to gravity and internally by collagen fibril and cellular retractive forces that create stress and energy equilibrium required for homeostasis. Mechanotransduction involves mechanical work (force through a distance) and energy storage as kinetic and potential energy. This leads to changes in cell mitosis or apoptosis and the synthesis or loss of tissue components. It involves the application of energy directly to cells through integrin-mediated processes, cell-cell connections, stretching of the cell cytoplasm, and activation of the cell nucleus via yes-associated protein (YAP) and transcriptional coactivator with PDZ-motif (TAZ). These processes involve numerous… More >

  • Open Access

    ARTICLE

    Biological function of miRNA-145-5p in angiotensin II induced renal inflammation

    BIN LI1,2,3,#, YUCHENG SHENG4,#, XIAOYING XU4, SHENGCUN WANG3, HONGYAN SONG3, JINGYUAN LI3, HAONAN JI1, QINGHUA WANG3, XIAODI ZHOU1,*, LONGJU QI2,*
    BIOCELL, DOI:10.32604/biocell.2024.047404
    Abstract Objective:: Chronic kidney disease (CKD) is a progressive disorder characterized by intricate structural and functional alterations in the kidneys, attributable to diverse causative factors. Notably, the therapeutic promise of miR-145-5p in addressing renal pathologies has been discerned. This investigation seeks to elucidate the functional role of miR-145-5p in injured kidneys by subjecting human glomerular mesangial cells (HGMCs) to stimulation with Angiotensin II (AngII). Materials and Methods:: Cellular viability and the levels of inflammatory mediators were evaluated utilizing Cell Counting Kit-8 (CCK-8), quantitative real-time polymerase chain reaction (qRT-PCR), and western blot methodologies, both in the presence of AngII incubation and in… More >

  • Open Access

    ARTICLE

    Identification prognostic features related to sphingolipid metabolism and experimental validation of TRIM47 in hepatocellular carcinoma

    JIAN TANG1, CHENQIANG ZHU1, YUN CHEN1, YUNLONG WU1, MING HE1, YI ZHOU2, MINGHUA XIE2,*
    BIOCELL, DOI:10.32604/biocell.2024.047562
    (This article belongs to this Special Issue: Navigating the Interplay of Cancer, Autophagy, ER Stress, Cell Cycle and Apoptosis: Mechanisms, Therapies, and Future Directions)
    Abstract Background: The specific impact of sphingolipid metabolism on developing hepatocellular Carcinoma (HCC) remains unclear. This study aims to explore the relationship between sphingolipid metabolism and HCC prognosis, immune response, and drug sensitivity. Methods: Data were obtained from The Cancer Genome Atlas (TCGA)-Hepatocellular Carcinoma (LIHC) and Gene Expression Omnibus (GEO, GSE14520 datasets). 47 sphingolipid metabolism genes were obtained from the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. After classifying HCC samples using the Non-negative Matrix Factorization (NMF) clustering method, differentially expressed genes were screened. Then, 8 risk genes were obtained by univariate analysis, survival random forest reduction and lasso analysis.… More >
    Graphic Abstract

    Identification prognostic features related to sphingolipid metabolism and experimental validation of TRIM47 in hepatocellular carcinoma

  • Open Access

    ARTICLE

    Amitriptyline inhibits NLRP3 inflammasome activation via the ASM/CE pathway in a cell model of NAFLD

    QIN LIU1,#, CHUNYAN NIU2,#,*, QIANG ZHANG3, SHIQIN SUN4, YUE CHEN2, YONGQIANG SHI2
    BIOCELL, DOI:10.32604/biocell.2024.048551
    Abstract Background: Nonalcoholic fatty liver disease (NAFLD) is a global health concern with the acid sphingomyelinase (ASM)/ceramide (CE) pathway and the NOD-like receptor family, pyrin domain-containing protein 3 (NLRP3) inflammasome identified as pivotal players in lipid disorders and inflammation. This study explores the interaction mechanism between the ASM/CE pathway and NLRP3 in NAFLD cell models, aiming to understand the impact of amitriptyline (Ami), an ASM inhibitor, on lipid deposition and hepatocyte injury by regulating the ASM/CE-NLRP3 pathway. Methods: HepG2 and HL-7702 cells were exposed to free fatty acids (FFAs) to establish the NAFLD model. The cells were divided into 5 groups:… More >

  • Open Access

    ARTICLE

    Knockdown of RCN1 contributes to the apoptosis of colorectal cancer via regulating IP3R1

    XUAN SHI1,2, YUFEN WANG1, CHENYU LI1, WANGSHU FU3, XINYUE ZHANG3, AIXIA GONG1,*
    BIOCELL, DOI:10.32604/biocell.2024.048076
    (This article belongs to this Special Issue: Navigating the Interplay of Cancer, Autophagy, ER Stress, Cell Cycle and Apoptosis: Mechanisms, Therapies, and Future Directions)
    Abstract Background: The incidence of colorectal cancer (CRC) has been increasing in recent years. Thus, the discovery of factors that can assist in alleviating CRC is urgently warranted. Methods: To identify a potential factor involved in the development of CRC, we screened the upregulated genes in tumor tissues through four datasets from an online database. The expression of reticulocalbin 1 (RCN1), a Ca2+-binding protein, was upregulated in the four datasets. Based on loss-of-function experiments, the effect of RCN1 on cell viability was assessed by Cell Counting Kit-8 (CCK-8) assay. The regulatory effect of RCN1 on apoptosis was evaluated through Annexin V-fluorescein… More >
    Graphic Abstract

    Knockdown of RCN1 contributes to the apoptosis of colorectal cancer via regulating IP3R1

  • Open Access

    ARTICLE

    Machine learning and bioinformatics to identify biomarkers in response to Burkholderia pseudomallei infection in mice

    YAO FANG1,2,#, FEI XIA1,#, FEIFEI TIAN3, LEI QU1, FANG YANG1, JUAN FANG1,2, ZHENHONG HU1,*, HAICHAO LIU1,*
    BIOCELL, DOI:10.32604/biocell.2024.031539
    (This article belongs to this Special Issue: Bioinformatics Study of Diseases)
    Abstract Objective: In the realm of Class I pathogens, Burkholderia pseudomallei (BP) stands out for its propensity to induce severe pathogenicity. Investigating the intricate interactions between BP and host cells is imperative for comprehending the dynamics of BP infection and discerning biomarkers indicative of the host cell response process. Methods: mRNA extraction from BP-infected mouse macrophages constituted the initial step of our study. Employing gene expression arrays, the extracted RNA underwent conversion into digital signals. The percentile shift method facilitated data processing, with the identification of genes manifesting significant differences accomplished through the application of the t-test. Subsequently, a comprehensive analysis… More >

  • Open Access

    ARTICLE

    CARD11 serves as a therapeutic biomarker for the drug therapies of ccRCC

    KAIWEN TIAN#, HANZHONG CHEN#, QIANQIAN WANG, FENGLIAN JIANG, CHUNXIANG FENG, TENG LI, XIAOYONG PU, YANLIN TANG*, JIUMIN LIU*
    BIOCELL, DOI:10.32604/biocell.2024.048737
    (This article belongs to this Special Issue: Frontiers in cancer: tumor microenvironment)
    Abstract Background: The incidence of clear cell renal cell carcinoma (ccRCC) is globally high; however, despite the introduction of innovative drug therapies, there remains a lack of effective biomarkers for evaluating treatment response. Recently, Caspase recruiting domain-containing protein 11 (CARD11) has garnered attention due to its significant association with tumor development and the immune system. Methods: The expression of CARD11 mRNA and protein in ccRCC were analyzed by public database and immunohistochemistry. The focus of this study is on the epigenomic modifications of CARD11, its expression of ccRCC immunophenotype, and its correlation with response to immunotherapy and targeted therapy. Furthermore, to… More >
    Graphic Abstract

    CARD11 serves as a therapeutic biomarker for the drug therapies of ccRCC

  • Open Access

    ARTICLE

    Galectin 2 regulates JAK/STAT3 signaling activity to modulate oral squamous cell carcinoma proliferation and migration in vitro

    XINRU FENG1, LI XIAO2,*
    BIOCELL, DOI:10.32604/biocell.2024.048395
    (This article belongs to this Special Issue: Subcellular Organelles and Cellular Molecules: Localization, Detection, Prediction, and Diseases)
    Abstract Background: Galectin 2 (LGALS2) is a protein previously reported to serve as a mediator of disease progression in a range of cancers. The function of LGALS2 in oral squamous cell carcinoma (OSCC), however, has yet to be explored, prompting the present study to address this literature gap. Methods: Overall, 144 paired malignant tumor tissues and paracancerous OSCC patient samples were harvested and the LGALS2 expression levels were examined through qPCR and western immunoblotting. The LGALS2 coding sequence was introduced into the pcDNA3.0 vector, to enable the overexpression of this gene, while an LGALS2-specific shRNA and corresponding controls were also obtained.… More >

  • Open Access

    REVIEW

    Non-coding RNA as future target for diagnose and treatment of perineural invasion in cancers

    BINGJIE LI1,#, WENBO CAO1,2,3,#, JINJING XIAO1, YIXIAO CHEN1, QIYING WEI1, MINGJIN YUE4, SAIJUN MO1,2,3,*
    BIOCELL, DOI:10.32604/biocell.2024.049160
    (This article belongs to this Special Issue: Advances in Biomarker Research: Unveiling the Pathways to Precision Medicine)
    Abstract Perineural invasion (PNI), a particularly insidious form of tumor metastasis distinct from hematogenous or lymphatic spread, has the capacity to extend well beyond the primary tumor site, infiltrating distant regions devoid of lymphatic or vascular structures. PNI often heralds a decrease in patient survival rates and is recognized as an indicator of an unfavorable prognosis across a variety of cancers. Despite its clinical significance, the underlying molecular mechanisms of PNI remain elusive, complicating the development of specific and efficacious diagnostic and therapeutic strategies. In the realm of cancer research, non-coding RNAs (ncRNAs) have attracted considerable attention due to their multifaceted… More >

  • Open Access

    REVIEW

    Exercise and exerkine upregulation: Brain-derived neurotrophic factor as a potential non-pharmacological therapeutic strategy for Parkinson’s disease

    VIRAAJ VISHNU PRASAD, JENNIFER SALLY SAMSON, VENKATACHALAM DEEPA PARVATHI*
    BIOCELL, DOI:10.32604/biocell.2024.048776
    (This article belongs to this Special Issue: Exploring the Cellular Mechanisms of Neurodegenerative Diseases)
    Abstract Physical activity and exercise have several beneficial roles in enhancing both physiological and psychological well-being of an individual. In addition to aiding the regulation of aerobic and anaerobic metabolism, exercise can stimulate the synthesis of exerkine hormones in the circulatory system. Among several exerkines that have been investigated for their therapeutic potential, Brain-derived neurotrophic factor (BDNF) is considered the most promising candidate, especially in the management of neurodegenerative diseases. Owing to the ability of physical activity to enhance BDNF synthesis, several experimental studies conducted so far have validated this hypothesis and produced satisfactory results at the pre-clinical level. This review… More >
    Graphic Abstract

    Exercise and exerkine upregulation: Brain-derived neurotrophic factor as a potential non-pharmacological therapeutic strategy for Parkinson’s disease

  • Open Access

    ARTICLE

    Inhibition of SLC26A4 regulated by electroacupuncture suppresses the progression of myocardial ischemia-reperfusion injury

    FEI KONG1, QIYUAN TIAN2, BINGLIN KUANG3, LILI SHANG4, XIAOXIAO ZHANG5, DONGYANG LI5, YING KONG6,*
    BIOCELL, DOI:10.32604/biocell.2024.046342
    (This article belongs to this Special Issue: Advances in Biomarker Research: Unveiling the Pathways to Precision Medicine)
    Abstract Introduction: Myocardial ischemia-reperfusion (IR) injury has received widespread attention due to its damaging effects. Electroacupuncture (EA) pretreatment has preventive effects on myocardial IR injury. SLC26A4 is a Na+ independent anion reverse transporter and has not been reported in myocardial IR injury. Objectives: To find potential genes that may be regulated by EA and explore the role of this gene in myocardial IR injury. Methods: RNA sequencing and bioinformatics analysis were performed to obtain the differentially expressed genes in the myocardial tissue of IR rats with EA pretreatment. Myocardial infarction size was detected by TTC staining. Serum CK, creatinine kinase-myocardial band,… More >
    Graphic Abstract

    Inhibition of SLC26A4 regulated by electroacupuncture suppresses the progression of myocardial ischemia-reperfusion injury

  • Open Access

    ARTICLE

    Bronchoalveolar lavage fluid metagenomic next-generation sequencing assay for identifying pathogens in lung cancer patients

    JIYU WANG1,2, HUIXIA LI1,2, DEYUAN ZHOU1,2, LIHONG BAI1,2, KEJING TANG1,2,3,*
    BIOCELL, DOI:10.32604/biocell.2024.030420
    (This article belongs to this Special Issue: Bioinformatics Study of Diseases)
    Abstract Background: For patients with lung cancer, timely identification of new lung lesions as infectious or non-infectious, and accurate identification of pathogens is very important in improving OS of patients. As a new auxiliary examination, metagenomic next-generation sequencing (mNGS) is believed to be more accurate in diagnosing infectious diseases in patients without underlying diseases, compared with conventional microbial tests (CMTs). We designed this study to find out whether mNGS has better performance in distinguishing infectious and non-infectious diseases in lung cancer patients using bronchoalveolar lavage fluid (BALF). Materials and Methods: This study was a real-world retrospective review based on electronic medical… More >

  • Open Access

    ARTICLE

    Gp78 regulates PMP22 and causes ER stress and autophagy in EV71-VP1-overexpressing mouse Schwann cells

    DANPING ZHU1,#, GUANGMING LIU1,#, KUAN FENG1, SUYUN LI1, DANDAN HU2, SIDA YANG3,*, PEIQING LI1,*
    BIOCELL, DOI:10.32604/biocell.2024.044856
    Abstract Background: During Enterovirus type 71 (EV71) infection, the structural viral protein 1 (VP1) activates endoplasmic reticulum (ER) stress associated with peripheral myelin protein 22 (PMP22) accumulation and induces autophagy. However, the specific mechanism behind this process remains elusive. Methods: In this research, we used the VP1-overexpressing mouse Schwann cells (SCs) models co-transfected with a PMP22 silencing or Autocrine motility factor receptor (AMFR/gp78) overexpressing vector to explore the regulation of gp78 on PMP22 and its relationship with autophagy and apoptosis. Results: The activity of gp78 could be influenced by EV71-VP1, leading to a decrease in the ubiquitination and degradation of PMP22,… More >

  • Open Access

    REVIEW

    Therapeutic and regenerative potential of different sources of mesenchymal stem cells for cardiovascular diseases

    YARA ALZGHOUL, HALA J. BANI ISSA, AHMAD K. SANAJLEH, TAQWA ALABDUH, FATIMAH RABABAH, MAHA AL-SHDAIFAT, EJLAL ABU-EL-RUB*, FATIMAH ALMAHASNEH, RAMADA R. KHASAWNEH, AYMAN ALZU’BI, HUTHAIFA MAGABLEH
    BIOCELL, DOI:10.32604/biocell.2024.048056
    (This article belongs to this Special Issue: Perspectives on Stem Cells and Regenerative Medicine)
    Abstract Mesenchymal stem cells (MSCs) are ideal candidates for treating many cardiovascular diseases. MSCs can modify the internal cardiac microenvironment to facilitate their immunomodulatory and differentiation abilities, which are essential to restore heart function. MSCs can be easily isolated from different sources, including bone marrow, adipose tissues, umbilical cord, and dental pulp. MSCs from various sources differ in their regenerative and therapeutic abilities for cardiovascular disorders. In this review, we will summarize the therapeutic potential of each MSC source for heart diseases and highlight the possible molecular mechanisms of each source to restore cardiac function. More >

  • Open Access

    ARTICLE

    miR-30a-5p/PHTF2 axis regulates the tumorigenesis and metastasis of lung adenocarcinoma

    LIJUAN ZHANG1,#, QINGYIN MENG2,#, LI ZHUANG1, QUAN GONG1, XIANDA HUANG3, XUEQIN LI1, SHIJUAN LI1, GUOQIN WANG4, XICAI WANG5,*
    BIOCELL, DOI:10.32604/biocell.2024.047260
    (This article belongs to this Special Issue: MicroRNA as Biomarkers for Disease Diagnosis and Progression)
    Abstract Background: Lung adenocarcinoma is a very pervasive histological form of lung cancers, and inhibiting metastasis is crucial for effective treatment. In this investigation, we explored the functional interaction of miR-30a-5p and the putative transcription factor 2 of the homeodomain (PHTF2) in dictating the aggressiveness and metastasis of lung adenocarcinoma. Method: We collected clinical samples to evaluate the expression patterns of miR-30a-5p and PHTF2 in lung adenocarcinoma along with normal tissues. Cellular experiments including cell count kit (CCK)-8 growth assay, apoptosis analysis, migration and invasion examinations were performed to assess the aggressiveness of lung adenocarcinoma cells. Furthermore, we examined tumorigenesis and… More >
    Graphic Abstract

    miR-30a-5p/PHTF2 axis regulates the tumorigenesis and metastasis of lung adenocarcinoma

  • Open Access

    ARTICLE

    Puerarin mediated miR-30b-5p targeting fibroblast activation protein against oral submucous fibrosis

    SAIFEI XIE1,2,4, HUI XIE2,*, JINCAI GUO3, JIN TAN4, YULIN YU1, MINYI ZHANG1, SHANG WEN1
    BIOCELL, DOI:10.32604/biocell.2024.046691
    Abstract Background: Puerarin (Pue) has been reported to be a natural active ingredient with multiple antifibrotic properties. This work aimed at exploring the function of Pue in oral submucous fibrosis (OSF) treatment. Methods: Human oral mucosa fibroblasts (hOMF) were induced with transforming growth factor beta1 (TGF-β1) and intervened with Pue. Expressions of fibrosis-related markers were analyzed by Western blot and IF staining. Cell viability was characterized by the CCK-8 assay. Expressions of miR-30 family members were quantified by qRT-PCR. The correlation between fibroblast activation protein (FAP) and miR-30 family expression was evaluated by the Pearson correlation coefficient. Bioinformatics prediction and dual-luciferase… More >
    Graphic Abstract

    Puerarin mediated miR-30b-5p targeting fibroblast activation protein against oral submucous fibrosis

  • Open Access

    REVIEW

    A perspective review on the biosynthesis of plant-based secondary metabolites and their application as potent drugs

    JHILAM PRAMANIK1,#, AKASH KUMAR2, SARVESH RUSTAGI3,#, MEHAK KATYAL4, SHEETAL THAKUR5, JUTISHNA BORA6, SUMIRA MALIK6,7,*, ANCHAL TREHAN8, NAYAN TALUKDAR9, PETR SLAMA10,*
    BIOCELL, DOI:10.32604/biocell.2023.029031
    Abstract Many phytochemicals and their derived metabolites produced by plants are extensively employed in commercial goods, pharmaceutical products as well as in the environmental and medical fields. However, these secondary metabolites obtained from plants are in low amounts, and it is difficult to synthesize them at the industrial level. Despite these challenges, they may be utilized for a variety of medicinal products that are either available in the market or are being researched and tested. Secondary metabolites are complex compounds that exhibit chirality. Further, under controlled conditions with elicitors, desired secondary metabolites may be produced from plant cell cultures. This review… More >
    Graphic Abstract

    A perspective review on the biosynthesis of plant-based secondary metabolites and their application as potent drugs