DONGMEI GAO^1,#, JIAOE CHEN^2,#, HONGZHANG LI^2,*, JUN ZHAO^3,*

BIOCELL, Vol.46, No.10, pp. 2275-2280, 2022, DOI:10.32604/biocell.2022.021008

Abstract Introduction: Human cytomegalovirus (HCMV) is reported to be involved in the occurrence of many human diseases. To further investigate the biological changes of HCMV, we analyzed the relevant factors that affect the autophagy caused by HCMV infection. Methods: Firstly, we cultured human embryonic lung fibroblasts (HELF) cells with HCMV infection, and evaluated the effects of HELF cells infected with different viruses through Enzyme-linked immunoabsorbent assay (ELISA), Real-time quantitative Polymerase Chain Reaction (RT-qPCR), Acridine orange (AO) staining and Western blotting (WB) experiments. Results: Through the above experiments, we found that the combined treatment of HCMV infection and carbamazepine, rapamycin and si-mTOR… More >

SHUAI LIU¹, JIANZHONG XU^1,*, TINGSHAN LIU¹, ZHIMING RAO², WEIGUO ZHANG^1,*

BIOCELL, Vol.46, No.10, pp. 2303-2313, 2022, DOI:10.32604/biocell.2022.020899

Abstract In this study, phosphoenolpyruvate and erythrose-4-phosphate are efficiently supplied by collaborative design of Embden-Meyerhof-Parnas (EMP) pathway and pentose phosphate (PP) pathway in Escherichia coli, thus increasing the L-tryptophan production. Firstly, the effects of disrupting EMP pathway on L-tryptophan production were studied, and the results indicated that the strain with deletion of phosphofructokinase A (i.e., E. coli JW-5 ΔpfkA) produced 23.4 ± 2.1 g/L of L-tryptophan production. However, deletion of phosphofructokinase A and glucosephosphate isomerase is not conducive to glucose consumption and cell growth, especially deletion of glucosephosphate isomerase. Next, the carbon flux in PP pathway was enhanced by introduction of… More >

MEIHUA CHEN^1,2, HUIHUI WANG³, XIAOFENG CHEN^1,2, YAN CHEN^1,2, TIANYING BIAN^1,2,*

BIOCELL, Vol.46, No.10, pp. 2281-2289, 2022, DOI:10.32604/biocell.2022.020697

Abstract Periodontitis is a frequent chronic inflammatory disorder destroying periodontium. Recent studies have revealed the role of bromodomain and extraterminal domain inhibitor (BETi) and microRNA (miR)-130a in regulating macrophage polarization and pro-inflammatory response. However, little is known about whether apabetalone (a novel BETi) and miR-130a are correlated with chronic inflammatory state in periodontitis by regulating macrophage polarization. Here murine RAW264.7 macrophages were applied as an in vitro inflammatory model. After treatment with Porphyromonas gingivalis-derived lipopolysaccharide (Pg LPS) and apabetalone, the expression of macrophage M1 polarization markers and inflammatory cytokines was assessed using real-time PCR, western blot, and enzyme-linked immuno sorbent assay… More >

HUILI ZHU¹, LINA XIAO¹, XIA YIN¹, SHIBING XIANG¹, CHUNHUI WANG^2,*

BIOCELL, Vol.46, No.10, pp. 2241-2256, 2022, DOI:10.32604/biocell.2022.020389

Abstract Pancreatic ductal adenocarcinoma (PDAC) is highly heterogeneous, making its prognosis prediction difficult. The arachidonic acid (AA) cascade is involved in carcinogenesis. Therefore, the metabolic enzymes of the AA cascade consist of lipoxygenases (LOXs), phospholipase A2s (PLA2s), and cyclooxygenases (COXs) along with their metabolic products, including leukotrienes. Nevertheless, the prognostic potential of AA metabolism-associated PDAC has not been explored. Herein, the mRNA expression patterns and the matching clinical information of individuals with PDAC were abstracted from online data resources. We employed the LASSO Cox regression model to develop a multigene clinical signature in the TCGA queue. The GEO queue and the… More >

HAIYANG CHEN^1,2,#, JINGYAO SU^1,#, DANYANG CHEN^1,#, YUYE DU¹, RUILIN ZHENG¹, QINGLIN DENG², QIANQIAN DU³, BING ZHU^1,*, YINGHUA LI^1,*

BIOCELL, Vol.46, No.10, pp. 2267-2273, 2022, DOI:10.32604/biocell.2022.020218

Abstract At present, Hepatocarcinoma is one of the main causes of tumor related death all over the world. However, there are still many clinical restrictions on the treatment of liver cancer. Recently, L-Selenocystine has been shown to be a novel treatment for tumors, especially human glioma cells. But, the mechanism of L-Selenocystine against hepatocellular carcinoma remains unclear. Therefore, the main objective of this study was to investigate the effects of L-Selenocystine on HepG2 cell proliferation and activation of reactive oxygen species (ROS) mediated signaling pathway. L-Selenocystine can significantly inhibit HepG2 cell proliferation by activating caspase-3 and cleaving PARP to induce apoptosis.… More >

CARL RANDALL HARRELL^1,#, ANA VOLAREVIC^2,#,*, DRAGICA PAVLOVIC³, VALENTIN DJONOV⁴, VLADISLAV VOLAREVIC^4,5,*

BIOCELL, Vol.46, No.10, pp. 2195-2200, 2022, DOI:10.32604/biocell.2022.020175

Abstract Detrimental immune response has a crucially important role in the development and progression of inflammatory eye diseases. Inflammatory mediators and proteolytic enzymes released by activated immune cells induce serious injury of corneal epithelial cells and retinal ganglion cell which may result in the vision loss. Mesenchymal stem cells (MSCs) are regulatory cells which produce various immunosuppressive factors that modulate phenotype and function of inflammatory immune cells. However, several safety issues, including undesired differentiation and emboli formation, limit clinical use of MSCs. MSC-derived exosomes (MSC-Exos) are nano-sized extracellular vesicles which contain all MSC-derived immunoregulatory factors. Intraocular administration of MSC-Exos efficiently attenuated… More >

KANIZ FATEMA, ZACHARY LARSON, JARED BARROTT^*

BIOCELL, Vol.46, No.10, pp. 2177-2193, 2022, DOI:10.32604/biocell.2022.020141

Abstract Osteosarcoma is one of the most genomically complex cancers and as result, it has been difficult to assign genomic aberrations that contribute to disease progression and patient outcome consistently across samples. One potential source for correlating osteosarcoma and genomic biomarkers is within the non-coding regions of RNA that are differentially expressed. However, it is unsurprising that a cancer classification that is fraught with genomic instability is likely to have numerous studies correlating non-coding RNA expression and function have been published on the subject. This review undertakes the formidable task of evaluating the published literature of non-coding RNAs in osteosarcoma. This… More >

KI-TAEK LIM^#,*, TEJAL V. PATIL^#, DINESH K. PATEL, SAYAN DEB DUTTA, KEYA GANGULY, AAYUSHI RANDHAWA

BIOCELL, Vol.46, No.10, pp. 2201-2208, 2022, DOI:10.32604/biocell.2022.020013

Abstract Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. Recently, MSCs have been widely explored for their application in regenerative medicine and COVID-19 treatment. Different approaches to evaluate the future of biomaterials and stem cell properties have been developed. However, misconceptions and ethical issues still exist, such as MSCs being non-angiogenic, anti-apoptotic, and immunoregulatory competencies. Embryonic stem cells isolation primarily requires the consent of donors and can include… More >

Yun Tan^1,2, Weizhao Wu², Ling Tan³, Haikuo Peng², Jiaohua Qin^2,*

Journal of New Media, Vol.4, No.3, pp. 155-164, 2022, DOI:10.32604/jnm.2022.031113

Abstract At present, segmentation for medical image is mainly based on fully supervised model training, which consumes a lot of time and labor for dataset labeling. To address this issue, we propose a semi-supervised medical image segmentation model based on a generative adversarial network framework for automated segmentation of arteries. The network is mainly composed of two parts: a segmentation network for medical image segmentation and a discriminant network for evaluating segmentation results. In the initial stage of network training, a fully supervised training method is adopted to make the segmentation network and the discrimination network have certain segmentation and discrimination… More >

Ziyi Wang¹, Xiaoyan Zhao^1,*, Hongjun Rong¹, Ying Tong¹, Jingang Shi²

Journal of New Media, Vol.4, No.3, pp. 145-153, 2022, DOI:10.32604/jnm.2022.030178

Abstract Microphone array-based sound source localization (SSL) is widely used in a variety of occasions such as video conferencing, robotic hearing, speech enhancement, speech recognition and so on. The traditional SSL methods cannot achieve satisfactory performance in adverse noisy and reverberant environments. In order to improve localization performance, a novel SSL algorithm using convolutional residual network (CRN) is proposed in this paper. The spatial features including time difference of arrivals (TDOAs) between microphone pairs and steered response power-phase transform (SRP-PHAT) spatial spectrum are extracted in each Gammatone sub-band. The spatial features of different sub-bands with a frame are combine into a… More >