Open Access
ARTICLE
Carbon Monoxide Signal Breaks Primary Seed Dormancy by Transcriptional Silence of DOG1 in Arabidopsis thaliana
Danni He, Guoli Deng, Songpei Ying, Wenjuan Yang, Jiali Wei, Ping Li*
Shanghai Key Laboratory of Bio-Energy Crops, Research Center for Natural Products, Plant Science Center, School of Life Sciences, Shanghai University, Shanghai, 200444, China
* Corresponding Author: Ping Li. Email:
Phyton-International Journal of Experimental Botany 2020, 89(3), 633-643. https://doi.org/10.32604/phyton.2020.010498
Received 07 March 2020; Accepted 10 April 2020; Issue published 22 June 2020
Abstract
Primary seed dormancy is an adaptive strategy that prevents germination for viable seeds in harsh environment, ensuring seeds germination under
favorable condition. Accurately inducing seeds germination in a controllable manner is important for crop production. Thus searching the chemicals that efficiently
breaks seed dormancy is valuable. DOG1 protein abundance in the freshly harvested seed is high, and its level is correlated to seed dormancy intensity, thus
DOG1 is regarded as the timer to evaluate the seed dormancy degree. In this
study, we found the carbon monoxide (CO) donor treatment, the transgenic line
with high CO content, showed lower seed dormancy, while scavenging CO, or
the mutant with lower CO level, presented strong primary seed dormancy, genetic
analysis showed that DOG1 was targeted by CO signal and was prerequisite for
CO-dependent seed dormancy release. Furthermore, we found CO signal activated the expression of ERF/AP2 transcriptional factor
ERF12, as well as
enhanced the binding of ERF12 to the promoter of
DOG1, ultimately transcriptional silence of
DOG1 expression to break primary seed dormancy. Meanwhile
CO signal reduced the histone acetylation level at the chromatin of
DOG1 locus
to suppress its expression. Together, our results revealed that CO acts as the novel
regulator to suppress
DOG1 expression and efficiently break primary seed dormancy through activating the negative factor ERF12.
Keywords
Cite This Article
He, D., Deng, G., Ying, S., Yang, W., Wei, J. et al. (2020). Carbon Monoxide Signal Breaks Primary Seed Dormancy by Transcriptional Silence of
DOG1 in
Arabidopsis thaliana.
Phyton-International Journal of Experimental Botany, 89(3), 633–643.
Citations