Open Access
ARTICLE
Influences of ascorbic acid and gibberellic acid in alleviating effects of salinity in Petunia under in vitro
Krupa-Małkiewicz M1, B Smolik2, M Sędzik2
1
Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin, Słowackiego str., 71-434 Szczecin, Poland.
2
Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology in Szczecin, Słowackiego str., 71-434 Szczecin, Poland.
Phyton-International Journal of Experimental Botany 2019, 88(1), 15-23. https://doi.org/10.32604/phyton.2019.04670
Abstract
Salinity is one of the abiotic stresses that limits the
growth and productivity of many crops. A possible survival strategy
for plant under saline conditions is to use compounds that could
minimize the harmful effects of salt stress on the plant development.
The objective of the presented study was to investigate the effect
of exogenous ascorbic acid (ASA) with or without gibberellic acid
(GA3) on key growth and biochemical parameters in two petunia
cultivars ‘Prism Rose’ and ‘Prism White’ under saline (150 mM
NaCl) and non-saline in vitro condition. Nodal cutting with an
axillary buds were used as explants. Application of 1 mM ascorbic
acid with or without 0.05 mM gibberellic acid into the MS medium
stimulated the length of shoots and the number of new shoots of
‘Prism Rose’; whereas, it decreased the root length and the number
of roots of both ‘Prism Rose’ and ‘Prism White’ under non-saline
condition. The addition of ascorbic acid with or without gibberellic
acid into the MS medium under saline condition, increased the
length of plants and the number of new shoots, but did not affect
their root number and length. NaCl treatments increased the
proline content and lipid peroxidation which was indicated by the
accumulation of malondialdehyde (MDA). The study revealed
a correlation between chlorophylls a and b content and the leaf
pigmentation intensity – parameter a*. Addition of 1 mM ascorbic
acid with 0.05 mM gibberellic acid into the MS medium plays
a protective role in salinity tolerance by improving the shoot
growth and the development as well as increasing the activities
of the antioxidant enzymes and other antioxidant substances.
Keywords
Cite This Article
M, K., Smolik, B., Sędzik, M. (2019). Influences of ascorbic acid and gibberellic acid in alleviating effects of salinity in Petunia under in vitro.
Phyton-International Journal of Experimental Botany, 88(1), 15–23. https://doi.org/10.32604/phyton.2019.04670