Vol.6, No.2, 2018, pp.169-175, doi:10.7569/JRM.2017.634151
OPEN ACCESS
ARTICLE
Saccharification of Sugarcane Bagasse Using an Enzymatic Extract Produced by Aspergillus fumigatus
  • Kênia F. R. Lamounier1, Patrisia O. Rodrigues1, Daniel Pasquini2*, Milla A. Baffi1
Institute of Agricultural Sciences, Federal University of Uberlândia (UFU), Uberlândia, MG, 38408-100, Brazil
Chemistry Institute, Federal University of Uberlândia (UFU), Uberlândia, MG, 38408-100, Brazil
*Corresponding author: daniel.pasquini@ufu.br
Abstract
This study investigates the efficiency of a crude enzymatic extract produced by Aspergillus fumigatus SCBM6 by solid state fermentation (SSF) in the hydrolysis of alkali pretreated sugarcane bagasse (PTB). After SSF using in natura sugarcane bagasse (SCB), the enzymatic extract presented 21.33 U.g–1 of β-glucosidase and 544.46 U.g–1of xylanase. The alkaline pretreatment with sodium hydroxide (2% NaOH (w/v) removed 43% of the lignin from PTB and the cellulosic fraction increased to 75%. The hydrolysis was optimized as a function of time, temperature, and concentration of PTB. After hydrolysis, the maximum yield (30.05%) of total released reducing sugars (TRS) was obtained under the following conditions: 24 h, 55 °C, 2% of PTB and 3 U.g–1 of β-glucosidase (CBU). Furthermore, an approximate TRS value (26.4%) was also obtained after saccharification carried out during 6 h, 55 °C, 4% of PTB and 1 CBU. These results indicate that hydrolysis can be performed in a short incubation period and with low enzymatic load for reasonable TRS release.
Keywords
A. fumigatus, enzymatic hydrolysis, sugarcane bagasse, alkaline pretreatment, solid state fermentation
Cite This Article
F., K., Rodrigues, P. O., Pasquini, D., Baffi, M. A. (2018). Saccharification of Sugarcane Bagasse Using an Enzymatic Extract Produced by Aspergillus fumigatus. Journal of Renewable Materials, 6(2), 169–175.