Open Access
ARTICLE
Association of TRIM22 with the type 1 interferon response during primary human cytomegalovirus infection in THP-1 macrophages
Wei LI, Huihui GAO, Ran TAO, Lifang LIU, Shiqiang SHANG*
Department of Clinical Laboratory, the Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center For Child Health, Hangzhou, 310052, China
* Address correspondence to: Shiqiang Shang,
BIOCELL 2019, 43(4), 285-291. https://doi.org/10.32604/biocell.2019.08177
Abstract
As a response factor of interferon, tripartite motif (TRIM) 22 was reported to exert antiviral activity against
viruses. In this study, THP-1 macrophages were infected with human cytomegalovirus (HCMV) to establish the HCMV
lytic infection model. The mRNA levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and interferonbeta
(IFN-β) were significantly up-regulated in THP-1 macrophages at different infection time and titers. Moreover,
for the first time, upregulation of TRIM22 expression was found during HCMV infection at both mRNA and protein
levels in THP-1 macrophages. Furthermore, IFN-β could induce TRIM22 expression in THP-1 macrophages or HCMV
infected THP-1 macrophages. Depletion of TRIM22 increased replication activity of HCMV with increasing of HCMV
titers and HCMV proteins. In conclusion, it is the first report that HCMV can induce TRIM22 activation through
IFN-β signaling and TRIM22 can suppress replication of HCMV in THP-1 macrophages.
Keywords
Cite This Article
LI, W., GAO, H., TAO, R., LIU, L., SHANG, S. (2019). Association of TRIM22 with the type 1 interferon response during primary human cytomegalovirus infection in THP-1 macrophages.
BIOCELL, 43(4), 285–291.